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CC BY-NC-ND 4.0 · Eur J Dent 2015; 09(03): 319-323
DOI: 10.4103/1305-7456.163222
Original Article
Dental Investigation Society

The influence of type 2 diabetes mellitus on salivary matrix metalloproteinase-8 levels and periodontal parameters: A study in an Indian population

Namita Gupta
1   Department of Periodontics and Community Dentistry, Dr. Z. A. Dental College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
,
Narinder Dev Gupta
1   Department of Periodontics and Community Dentistry, Dr. Z. A. Dental College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
,
Akash Gupta
2   Department of Biochemistry, Subharti Medical College, Meerut, Uttar Pradesh, India
,
Lata Goyal
1   Department of Periodontics and Community Dentistry, Dr. Z. A. Dental College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
,
Sagar Garg
3   Department of Periodontics, Tamil Nadu Government Medical College, Chennai, Tamil Nadu, India
› Author Affiliations
Further Information

Publication History

Publication Date:
04 September 2019 (online)

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ABSTRACT

Objective: Although many studies reported more severe periodontal disease and the existing proinflammatory conditions in patients with diabetes but only few have examined the effect of type 2 diabetes mellitus (DM) on salivary matrix metalloproteinase-8 (MMP-8) level and other periodontal parameters. This study aims to evaluate the effect of type 2 DM on salivary MMP-8 levels and periodontal parameters, which might be useful in monitoring periodontal disease in diabetes. Materials and Methods: A total of 90 subjects were selected for the study and were divided into three groups: Group I included 30 healthy subjects; Group II included 30 subjects without type 2 DM but with chronic periodontitis, and Group III included 30 subjects with type 2 DM and chronic periodontitis. Periodontal parameters such as plaque index (PI), gingival index (GI), pocket probing depth (PPD), and clinical attachment level (CAL) were taken. The salivary MMP-8 level was estimated by Quantikine Human total MMP-8 immunoassay kit using ELISA method. Results: The mean value of the salivary MMP-8 of Group III was highest followed by Group II and Group I, the least. The other periodontal parameters PI, GI, PPD, CAL, was comparatively highest for Group III. Conclusion: This study suggests that diabetes is associated with an increased prevalence, extent, and severity of periodontitis. Furthermore, the increased levels of MMP-8 indicate the influence of diabetes on their salivary concentration.

Key words:

Chronic periodontitis - matrix metalloproteinase-8 - periodontal parameters - saliva - type 2 diabetes mellitus
 

  • REFERENCES

  • 1 Nunn ME. Understanding the etiology of periodontitis: An overview of periodontal risk factors. Periodontol 2000 2003; 32: 11-23
    CrossrefPubMedGoogle Scholar
  • 2 Seymour GJ, Gemmell E, Reinhardt RA, Eastcott J, Taubman MA. Immunopathogenesis of chronic inflammatory periodontal disease: Cellular and molecular mechanisms. J Periodontal Res 1993; 28: 478-86
    CrossrefPubMedGoogle Scholar
  • 3 Shlossman M, Knowler WC, Pettitt DJ, Genco RJ. Type 2 diabetes mellitus and periodontal disease. J Am Dent Assoc 1990; 121: 532-6
    CrossrefPubMedGoogle Scholar
  • 4 Mealey BL, Ocampo GL. Diabetes mellitus and periodontal disease. Periodontol 2000 2007; 44: 127-53
    CrossrefPubMedGoogle Scholar
  • 5 Löe H. Periodontal disease. The sixth complication of diabetes mellitus. Diabetes Care 1993; 16: 329-34
    CrossrefPubMedGoogle Scholar
  • 6 Schmidt AM, Hori O, Cao R, Yan SD, Brett J, Wautier JL. et al. RAGE: A novel cellular receptor for advanced glycation end products. Diabetes 1996; 45: S77-80
    CrossrefPubMedGoogle Scholar
  • 7 Ryan ME, Ramamurthy NS, Sorsa T, Golub LM. MMP-mediated events in diabetes. Ann N Y Acad Sci 1999; 878: 311-34
    CrossrefPubMedGoogle Scholar
  • 8 Nagase H, Woessner Jr JF. Matrix metalloproteinases. J Biol Chem 1999; 274: 21491-4
    CrossrefPubMedGoogle Scholar
  • 9 Bertoldi C, Lalla M, Pradelli JM, Cortellini P, Lucchi A, Zaffe D. Risk factors and socioeconomic condition effects on periodontal and dental health: A pilot study among adults over 50 years of age. Eur J Dent 2013; 7: 336-46
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Wahlgren J, Maisi P, Sorsa T, Sutinen M, Tervahartiala T, Pirilä E. et al. Expression and induction of collagenases (MMP-8 and -13) in plasma cells associated with bone-destructive lesions. J Pathol 2001; 194: 217-24
    CrossrefPubMedGoogle Scholar
  • 11 Moilanen M, Pirilä E, Grénman R, Sorsa T, Salo T. Expression and regulation of collagenase-2 (MMP-8) in head and neck squamous cell carcinomas. J Pathol 2002; 197: 72-81
    CrossrefPubMedGoogle Scholar
  • 12 Krupinski J, Turu MM, Font MA, Ahmed N, Sullivan M, Rubio F. et al. Increased tissue factor, MMP-8, and D-dimer expression in diabetic patients with unstable advanced carotid atherosclerosis. Vasc Health Risk Manag 2007; 3: 405-12
    PubMedGoogle Scholar
  • 13 Fine DH, Mandel ID. Indicators of periodontal disease activity: An evaluation. J Clin Periodontol 1986; 13: 533-46
    CrossrefPubMedGoogle Scholar
  • 14 Giannobile WV, Beikler T, Kinney JS, Ramseier CA, Morelli T, Wong DT. Saliva as a diagnostic tool for periodontal disease: Current state and future directions. Periodontol 2000 2009; 50: 52-64
    CrossrefPubMedGoogle Scholar
  • 15 Kraft-Neumärker M, Lorenz K, Koch R, Hoffmann T, Mäntylä P, Sorsa T. et al. Full-mouth profile of active MMP-8 in periodontitis patients. J Periodontal Res 2012; 47: 121-8
    CrossrefPubMedGoogle Scholar
  • 16 Sorsa T, Tjäderhane L, Konttinen YT, Lauhio A, Salo T, Lee HM. et al. Matrix metalloproteinases: Contribution to pathogenesis, diagnosis and treatment of periodontal inflammation. Ann Med 2006; 38: 306-21
    CrossrefPubMedGoogle Scholar
  • 17 Page RC, Eke PI. Case definitions for use in population-based surveillance of periodontitis. J Periodontol 2007; 78: 1387-99
    CrossrefPubMedGoogle Scholar
  • 18 Silness J, Loe H. Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condtion. Acta Odontol Scand 1964; 22: 121-35
    CrossrefPubMedGoogle Scholar
  • 19 Loe H, Silness J. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand 1963; 21: 533-51
    CrossrefPubMedGoogle Scholar
  • 20 Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci 1993; 694: 72-7
    CrossrefPubMedGoogle Scholar
  • 21 Cifcibasi E, Kantarci A, Badur S, Issever H, Cintan S. Impact of metronidazole and amoxicillin combination on matrix metalloproteinases-1 and tissue inhibitors of matrix metalloproteinases balance in generalized aggressive periodontitis. Eur J Dent 2015; 9: 53-9
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Mealey BL, Oates TW. American academy of periodontology. Diabetes mellitus and periodontal diseases. J Periodontol 2006; 77: 1289-303
    CrossrefPubMedGoogle Scholar
  • 23 Campus G, Salem A, Uzzau S, Baldoni E, Tonolo G. Diabetes and periodontal disease: A case-control study. J Periodontol 2005; 76: 418-25
    CrossrefPubMedGoogle Scholar
  • 24 Collin HL, Uusitupa M, Niskanen L, Kontturi-Närhi V, Markkanen H, Koivisto AM. et al. Periodontal findings in elderly patients with non-insulin dependent diabetes mellitus. J Periodontol 1998; 69: 962-6
    CrossrefPubMedGoogle Scholar
  • 25 Kiran M, Arpak N, Unsal E, Erdogan MF. The effect of improved periodontal health on metabolic control in type 2 diabetes mellitus. J Clin Periodontol 2005; 32: 266-72
    CrossrefPubMedGoogle Scholar
  • 26 Cutler CW, Machen RL, Jotwani R, Iacopino AM. Heightened gingival inflammation and attachment loss in type 2 diabetics with hyperlipidemia. J Periodontol 1999; 70: 1313-21
    CrossrefPubMedGoogle Scholar
  • 27 Ervasti T, Knuuttila M, Pohjamo L, Haukipuro K. Relation between control of diabetes and gingival bleeding. J Periodontol 1985; 56: 154-7
    CrossrefPubMedGoogle Scholar
  • 28 Alba-Loureiro TC, Munhoz CD, Martins JO, Cerchiaro GA, Scavone C, Curi R. et al. Neutrophil function and metabolism in individuals with diabetes mellitus. Braz J Med Biol Res 2007; 40: 1037-44
    PubMedGoogle Scholar
  • 29 Gursoy UK, Könönen E, Huumonen S, Tervahartiala T, Pussinen PJ, Suominen AL. et al. Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis. J Clin Periodontol 2013; 40: 18-25
    CrossrefPubMedGoogle Scholar
  • 30 Lalla E, Lamster IB, Stern DM, Schmidt AM. Receptor for advanced glycation end products, inflammation, and accelerated periodontal disease in diabetes: Mechanisms and insights into therapeutic modalities. Ann Periodontol 2001; 6: 113-8
    CrossrefPubMedGoogle Scholar
  • 31 Costa PP, Trevisan GL, Macedo GO, Palioto DB, Souza SL, Grisi MF. et al. Salivary interleukin-6, matrix metalloproteinase-8, and osteoprotegerin in patients with periodontitis and diabetes. J Periodontol 2010; 81: 384-91
    CrossrefPubMedGoogle Scholar
  • 32 Collin HL, Sorsa T, Meurman JH, Niskanen L, Salo T, Rönkä H. et al. Salivary matrix metalloproteinase (MMP-8) levels and gelatinase (MMP-9) activities in patients with type 2 diabetes mellitus. J Periodontal Res 2000; 35: 259-65
    CrossrefPubMedGoogle Scholar
  • 33 Kumar MS, Vamsi G, Sripriya R, Sehgal PK. Expression of matrix metalloproteinases (MMP-8 and -9) in chronic periodontitis patients with and without diabetes mellitus. J Periodontol 2006; 77: 1803-8
    CrossrefPubMedGoogle Scholar
  • 34 Rathnayake N, Akerman S, Klinge B, Lundegren N, Jansson H, Tryselius Y. et al. Salivary biomarkers for detection of systemic diseases. PLoS One 2013; 8: e61356
    CrossrefPubMedGoogle Scholar
  • 35 Miller CS, King Jr CP, Langub MC, Kryscio RJ, Thomas MV. Salivary biomarkers of existing periodontal disease: A cross-sectional study. J Am Dent Assoc 2006; 137: 322-9
    CrossrefPubMedGoogle Scholar
  • 36 Deshpande K, Jain A, Sharma R, Prashar S, Jain R. Diabetes and periodontitis. J Indian Soc Periodontol 2010; 14: 207-12
    CrossrefPubMedGoogle Scholar