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Semin Thromb Hemost 2001; 27(5): 473-482
DOI: 10.1055/s-2001-17958
Copyright © 2001 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Combined Quantitative 1H and 13C Nuclear Magnetic Resonance Spectroscopy for Characterization of Heparin Preparations

Marco Guerrini, Antonella Bisio, Giangiacomo Torri
  • ``G. Ronzoni'' Institute for Chemical and Biochemical Research, Milan, Italy
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Publication History

Publication Date:
22 October 2001 (online)

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ABSTRACT

The sulfation patterns of pig and bovine mucosal commercial heparin preparations can be characterized and distinguished from each other easily by analysis of their monodimensional proton and carbon nuclear magnetic resonance (1H and 13C-NMR) spectra. NMR spectroscopy can detect and quantify signals associated with major sequences as well as with minor residues such as the typical ones associated with the antithrombin (AT) binding sequence and the ``linkage region.'' Contaminants arising from industrial preparation processes are also detectable.

KEYWORD

Heparin - NMR spectra - sulfation patterns - antithrombin binding sequence

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