This study details the development and validation of a measure of mindful self-care, the Mindful Self-Care Scale (MSCS). Based on theory and emerging empirical work, the MSCS items were developed to align with a set of actionable practices that promote positive embodiment and well-being. The extant literature on self-care was reviewed and a set of items developed. Following expert review, the retained items were factor analyzed (N = 448, aged 18–71, 79.7% female, 90.0% Caucasian) resulting in six factors: physical care, supportive relationships, mindful awareness, self-compassion and purpose, mindful relaxation, and supportive structure. Overall, the MSCS was significantly and positively correlated with body esteem and negatively correlated with eating disorder risk. Confirmatory factor analyses were applied to a second independent sample (N = 452, aged 18–78, 69.7% female, 70.8% Caucasian) providing cross-validation of the six-dimensional structure of the MSCS. Internal consistency was upheld for the total scale and subscales. Findings and implications for future research follow along with a review of the limitations of this study.