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Models for Gain-of-Function and Loss-of-Function of MMPs

Transgenic and Gene Targeted Mice

  • Protocol
Matrix Metalloproteinase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 151))

Abstract

The most powerful approach for studying gene function in an intact animal is to regulate the levels of the gene product and thereby see gains-of-function or losses-of-function. The occasional mutation in the genes for the matrix metalloproteinases or their inhibitors, or polymorphism in their promoters that alter transcriptional regulation has been identified in humans and has helped define the function of these proteins. With ever increasing sophistication in producing targeted mutations in mice, there are now available null mutation in most of the known genes for the matrix metalloproteinases and their inhibitors. A number of mouse strains with ectopic expression of normal and mutant proteins have also been made. These transgenic mice are giving us new insights into the processes of development and pathogenesis.

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Author information

Authors and Affiliations

  1. Hormone Research Institute, University of California, San Francisco, CA

    Lisa M. Coussens

  2. Department of Pediatrics, Washington University School of Medicine, St. Louis, MO

    Steven D. Shapiro

  3. Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY

    Paul D. Soloway

  4. Department of Anatomy, University of California, San Francisco, CA

    Zena Werb

Authors
  1. Lisa M. Coussens
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  2. Steven D. Shapiro
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  3. Paul D. Soloway
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  4. Zena Werb
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Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich, UK

    Ian M. Clark PhD

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© 2001 Humana Press Inc.

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Coussens, L.M., Shapiro, S.D., Soloway, P.D., Werb, Z. (2001). Models for Gain-of-Function and Loss-of-Function of MMPs. In: Clark, I.M. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology™, vol 151. Humana Press. https://doi.org/10.1385/1-59259-046-2:149

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  • DOI: https://doi.org/10.1385/1-59259-046-2:149

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-733-5

  • Online ISBN: 978-1-59259-046-9

  • eBook Packages: Springer Protocols

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