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Mouse Testing Methods in Psychoneuroimmunology: An Overview of How to Measure Sickness, Depressive/Anxietal, Cognitive, and Physical Activity Behaviors

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Psychoneuroimmunology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 934))

Abstract

The field of psychoneuroimmunology (PNI) aims to uncover the processes and consequences of nervous, immune, and endocrine system relationships. Behavior is a consequence of such interactions and manifests from a complex interweave of factors including immune-to-neural and neural-to-immune communication. Often the signaling molecules involved during a particular episode of neuroimmune activation are not known but behavioral response provides evidence that bioactives such as neurotransmitters and cytokines are perturbed. Immunobehavioral phenotyping is a first-line approach when examining the neuroimmune system and its reaction to immune stimulation or suppression. Behavioral response is significantly more sensitive than direct measurement of a single specific bioactive and can quickly and efficiently rule in or out relevance of a particular immune challenge or therapeutic to neuroimmunity. Classically, immunobehavioral research was focused on sickness symptoms related to bacterial infection but neuroimmune activation is now a recognized complication of diseases and disorders ranging from cancer to diabesity. Immunobehaviors include lethargy, loss of appetite, and disinterest in social activity and the surrounding environment. In addition, neuroimmune activation can precipitate feelings of depression and anxiety while negatively impacting cognitive function and physical activity. Provided is a detailed overview of behavioral tests frequently used to examine neuroimmune activation in mice with a special emphasis on preexperimental conditions that can confound or prevent successful immunobehavioral experimentation.

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This research was supported by the National Institutes of Health (DK064862, NS058525 and AA019357 to G.G.F.), USDA National Institute of Food and Agriculture, Hatch project #ILLU-971-32.

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Authors and Affiliations

  1. Department of Animal Sciences, University of Illinois, Urbana, IL, USA

    Jason M. York

  2. Department of Pathology, College of Medicine, University of Illinois, Urbana, IL, USA

    Neil A. Blevins

  3. Department of Kinesiology and Community Health, University of Illinois, Urbana, IL, USA

    Tracy Baynard

  4. Department of Pathology, College of Medicine, University of Illinois at Urbana Champaign, Urbana, IL, USA

    Gregory G. Freund

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  1. Jason M. York
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  2. Neil A. Blevins
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  3. Tracy Baynard
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  4. Gregory G. Freund
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Correspondence to Gregory G. Freund .

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  1. PharmTao, Lafayette St 4601, Santa Clara, 95056, California, USA

    Qing Yan

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York, J.M., Blevins, N.A., Baynard, T., Freund, G.G. (2012). Mouse Testing Methods in Psychoneuroimmunology: An Overview of How to Measure Sickness, Depressive/Anxietal, Cognitive, and Physical Activity Behaviors. In: Yan, Q. (eds) Psychoneuroimmunology. Methods in Molecular Biology, vol 934. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-071-7_13

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  • DOI: https://doi.org/10.1007/978-1-62703-071-7_13

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