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Storage of Cell Lines

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 731))

Abstract

The successful storage of cell lines depends upon many factors, including the condition of the cells to be frozen and the experience of the operator. Attempting to freeze down unhealthy, contaminated or poorly labelled cells can have huge implications for a research laboratory. This chapter outlines the importance of good record keeping, vigilant monitoring, aseptic technique, and high-quality reagents in the successful storage and downstream propagation of cell lines.

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References

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Correspondence to Katharine A. Parker .

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© 2011 Springer Science+Business Media, LLC

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Parker, K.A. (2011). Storage of Cell Lines. In: Cree, I. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 731. Humana Press. https://doi.org/10.1007/978-1-61779-080-5_3

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  • DOI: https://doi.org/10.1007/978-1-61779-080-5_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-079-9

  • Online ISBN: 978-1-61779-080-5

  • eBook Packages: Springer Protocols

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