Abstract
During the past 40 years, mouse chimeras have served as invaluable tools for studying not only genetics but also embryonic development, and the path from undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows highlighting the most commonly used as well as more recently developed applications of various cell types and origins used in the production of chimeras. Finally, detailed protocols and trouble-shooting suggestions will be presented for each of the steps involved.
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Tanaka, M., Hadjantonakis, AK., Vintersten, K., Nagy, A. (2009). Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos. In: Wurst, W., Kühn, R. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 530. Humana Press. https://doi.org/10.1007/978-1-59745-471-1_15
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DOI: https://doi.org/10.1007/978-1-59745-471-1_15
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