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ES Cell Line Establishment

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Gene Knockout Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 530))

Abstract

A method is described to establish mouse embryonic stem cell (ESC) lines from hybrid and inbred strains of mice. Attention is paid not only to the methodology for isolation and culture but also to the validation of freshly derived lines, in order to be maintained for prolonged time without significant differentiation or karyotype instability, and to provide reproducible germline transmission in chimaeric mice.

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  1. Department of Applied Genetics, TaconicArtemis GmbH, Cologne, Germany

    Heidrun Kern & Branko Zevnik

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  1. Heidrun Kern
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  2. Branko Zevnik
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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Kern, H., Zevnik, B. (2009). ES Cell Line Establishment. In: Wurst, W., Kühn, R. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 530. Humana Press. https://doi.org/10.1007/978-1-59745-471-1_10

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    • DOI: https://doi.org/10.1007/978-1-59745-471-1_10

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-26-8

    • Online ISBN: 978-1-59745-471-1

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