Abstract
Proteomics is the large-scale analysis of proteins rendered possible by modern mass spectrometry analysis methods capable of identifying thousands of peptides/proteins in a fast high-throughput manner. Here I describe protocols for the preparation of fungal culture protein samples for mass spectrometry-based proteomics analysis including protein sample cleanup, proteolytic digestion, LC-MS/MS separation, and database search protein identification.
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Acknowledgment
I am grateful for the critical review of the manuscript by Sylvester Palys and the technical drawing of Fig. 2 by Denis Legault. The author is supported by Genome Canada and Génome Québec.
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Di Falco, M.R. (2018). Mass Spectrometry-Based Proteomics. In: de Vries, R., Tsang, A., Grigoriev, I. (eds) Fungal Genomics. Methods in Molecular Biology, vol 1775. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7804-5_9
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DOI: https://doi.org/10.1007/978-1-4939-7804-5_9
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