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Methods for Genomic Characterization and Maintenance of Anaerobic Fungi

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Fungal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1775))

Abstract

The rapid development of molecular biology and bioinformatics has fueled renewed interests in anaerobic fungi from the phylum Neocallimastigomycota. This chapter presents well-established methods for isolation, routine cultivation, and cryopreservation of anaerobic fungi. Moreover, detailed nucleic acid extraction protocols are provided, which should enable readers to isolate high-quality DNA and RNA from a variety of anaerobic fungal culture media for downstream applications such as next-generation sequencing.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of California, Santa Barbara, CA, USA

    Xuefeng Peng, Candice L. Swift & Michelle A. O’Malley

  2. Animal Production, Welfare and Veterinary Sciences, Harper Adams University, Newport, Shropshire, UK

    Michael K. Theodorou

Authors
  1. Xuefeng Peng
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  2. Candice L. Swift
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  3. Michael K. Theodorou
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  4. Michelle A. O’Malley
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Corresponding author

Correspondence to Michelle A. O’Malley .

Editor information

Editors and Affiliations

  1. Fungal Physiology, Westerdijk Fungal, Biodiversity Institute, Utrecht, The Netherlands

    Ronald P. de Vries

  2. Centre for Structural and Functional Genomics, Concordia University, Montreal, Québec, Canada

    Adrian Tsang

  3. US Department of Energy, Joint Genome Institute, Walnut Creek, California, USA

    Igor V. Grigoriev

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Peng, X., Swift, C.L., Theodorou, M.K., O’Malley, M.A. (2018). Methods for Genomic Characterization and Maintenance of Anaerobic Fungi. In: de Vries, R., Tsang, A., Grigoriev, I. (eds) Fungal Genomics. Methods in Molecular Biology, vol 1775. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7804-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7804-5_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7803-8

  • Online ISBN: 978-1-4939-7804-5

  • eBook Packages: Springer Protocols

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