CC BY-NC-ND 4.0 · Eur J Dent 2015; 09(03): 411-414
DOI: 10.4103/1305-7456.163225
Original Article
Dental Investigation Society

Antimicrobial activity of a temporary sealant used in endodontic treatment: An in vitro study

Helena Baruffaldi Domingos
1   Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil
,
Lucio Souza Gonçalves
1   Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil
,
Milton de Uzeda
1   Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
04 September 2019 (online)

ABSTRACT

Objective: The present study is aimed to evaluate the antimicrobial action of Coltosol® in direct contact with human saliva. Materials and Methods: Twelve different individuals were selected. Saliva samples were evaluated at four different time periods: Baseline 1 (T1-initial control), T2 (2 h), T4 (24 h after contact with a standardized sample of a coronary sealer) and baseline 2 (T3-fi nal control). Seeded plates were incubated at 37°C in a bacterial incubator for a period of 48–72 h. After the incubation period, the colony forming units were counted, and the results compared. Results: Differences were statistically significant. There was an inhibition of bacterial growth after the fi rst 2 h of contact and an increase in the number of bacteria after 24 h of direct contact between the material and the saliva. Coltosol® presented bacterial growth inhibition in direct contact with saliva. This inhibitory effect tended to decrease over time, as shown by the two periods when the material was in contact with different samples of saliva. Conclusions: The antimicrobial activity of the material is an important feature; however, other physical and chemical properties of the coronary temporary sealer should be considered.

 
  • REFERENCES

  • 1 Nóbrega LM, Delboni MG, Martinho FC, Zaia AA, Ferraz CC, Gomes BP. Treponema diversity in root canals with endodontic failure. Eur J Dent 2013; 7: 61-8
  • 2 Al-Hezaimi K, Naghshbandi J, Oglesby S, Simon JH, Rotstein I. Human saliva penetration of root canals obturated with two types of mineral trioxide aggregate cements. J Endod 2005; 31: 453-6
  • 3 Heling I, Gorfil C, Slutzky H, Kopolovic K, Zalkind M, Slutzky-Goldberg I. Endodontic failure caused by inadequate restorative procedures: Review and treatment recommendations. J Prosthet Dent 2002; 87: 674-8
  • 4 Pai SF, Yang SF, Sue WL, Chueh LH, Rivera EM. Microleakage between endodontic temporary restorative materials placed at different times. J Endod 1999; 25: 453-6
  • 5 Khayat A, Lee SJ, Torabinejad M. Human saliva penetration of coronally unsealed obturated root canals. J Endod 1993; 19: 458-61
  • 6 Siqueira Jr JF. editors. Endodontic infections and the etiology of apical periodontitis. In: Treatment of Endodontic Infections. Rio de Janeiro: Quintessence; 2011: P. 7-15
  • 7 Vigodent SA. Available from: http://www.vigodent.com.br/produto/?CodProduto=17 [Last accessed on 2013 Dec 02]
  • 8 Couto PH, Pinheiro JM, Couto LH, Freiras MR. In vitro evaluation of coronal microleakage of five materials. Arq Bras Odontol 2010; 6: 78-88
  • 9 Reiss-Araújo C, Gominho L, Albuquerque DS, Cardoso JC, Macedo GM, Jesus RCB. et al. The antimicrobial action analysis of coronary temporary sealing materials used in endodontics. RSBO 2006; 3: 7-14
  • 10 Siqueira Jr JF, Lopes HP. editors. Intracanal medication. In: Endodontics: Biology and technique. Rio de Janeiro: Guanabara Koogan; 2010: P. 611-3
  • 11 Cruz EV, Shigetani Y, Ishikawa K, Kota K, Iwaku M, Goodis HE. A laboratory study of coronal microleakage using four temporary restorative materials. Int Endod J 2002; 35: 315-20
  • 12 Zmener O, Banegas G, Pameijer CH. Coronal microleakage of three temporary restorative materials: An in vitro study. J Endod 2004; 30: 582-4
  • 13 Grillo JP, Uzeda M, Alves FR, Goncalves LS. Temporary coronary dental sealers: Determination of in vitro antimicrobial activity. Rev Assoc Paul Cir Dent 2013; 67: 136-40
  • 14 Aledrissy HI, Abubakr NH, Ahmed YahiaN, Eltayib IbrahimY. Coronal microleakage for readymade and hand mixed temporary filling materials. Iran Endod J 2011; 6: 155-9
  • 15 Madarati A, Rekab MS, Watts DC, Qualtrough A. Time-dependence of coronal seal of temporary materials used in endodontics. Aust Endod J 2008; 34: 89-93
  • 16 Magura ME, Kafrawy AH, Brown Jr CE, Newton CW. Human saliva coronal microleakage in obturated root canals: An in vitro study. J Endod 1991; 17: 324-31
  • 17 Naseri M, Ahangari Z, Shahbazi MoghadamM, Mohammadian M. Coronal sealing ability of three temporary filling materials. Iran Endod J 2012; 7: 20-4
  • 18 Srikumar GP, Varma KR, Shetty KH, Kumar P. Coronal microleakage with five different temporary restorative materials following walking bleach technique: An ex-vivo study. Contemp Clin Dent 2012; 3: 421-6