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CC BY 4.0 · Eur J Dent 2024; 18(01): 297-303
DOI: 10.1055/s-0043-1768468
Original Article

Preliminary Study on the Expression of CLLD7 and CHC1L Proteins in Oral Squamous Cell Carcinoma

Boworn Klongnoi
1   Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Bishwa Prakash Bhattarai 
1   Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
2   Department of Clinical Dentistry, Walailak University International College of Dentistry, Walailak University, Bangkok, Thailand
,
Rachai Juengsomjit
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Ounruean Meesakul
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Sopee Poomsawat
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Kajohnkiart Janebodin
4   Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Siribang-on Piboonniyom Khovidhunkit 
5   Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
› Author Affiliations Funding This research is supported by a grant from the Faculty of Dentistry, Mahidol University to Boworn Klongnoi (2018).
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Abstract

Objective This study aimed to preliminarily evaluate the expression of two putative tumor suppressor proteins, including chronic lymphocytic leukemia deletion gene 7 (CLLD7) and chromosome condensation 1-like (CHC1L) proteins in oral squamous cell carcinoma (OSCC).

Materials and Methods Expression of CLLD7 and CHC1L proteins was analyzed in 19 OSCC and 12 normal oral mucosa (NOM) using immunohistochemistry. The percentage of positive cells and intensity of staining were semiquantitatively assessed and expressed with an immunoreactive score. The number of positive cells at various subcellular localizations was evaluated and presented in percentages. The immunoreactivity scores and percentages of positive cells at various localizations were compared between the normal and OSCC groups with statistical significance at p-value less than 0.05.

Results According to immunohistochemical analysis, the immunoreactivity scores for both CLLD7 and CHC1L were higher in NOM than those of OSCC. Analysis of CLLD7 localization revealed predominant nuclear staining at basal and parabasal areas in NOM, whereas more cytoplasmic staining was observed in OSCC. For CHC1L, nuclear staining was prominent in NOM. In contrast, significantly increased plasma membrane staining was detected in OSCC.

Conclusion The expression of CLLD7 and CHC1L proteins was reduced in OSCC. Alterations in the subcellular localization of these two proteins in OSCC were also demonstrated. These preliminary results suggest that CLLD7 and CHC1L are aberrantly expressed in OSCC. The precise mechanisms of these putative tumor suppressor proteins in OSCC require future studies.

Keywords

CLLD7 - CHC1L - immunohistochemistry - oral squamous cell carcinoma - tumor suppressor proteins

Authors' Contributions

BK contributed to funding acquisition and resources; BPB helped in investigation, data curation, formal analysis, and writing—original draft preparation; RJ was involved in investigation; OM and KJ helped in resources; SP contributed to formal analysis and supervision; SPK helped in conceptualization, experimental designs, supervision, and writing—manuscript design and final draft.


Institutional Review Board Statement

The study was performed in accordance with the principles of the Declaration of Helsinki. This study was approved by the Faculty of Dentistry/Faculty of Pharmacy, Mahidol University, Institutional Review Board (COE.No.MU-DT/PY-IRB 2018/025.1106).


Data Availability Statement

The data presented in this study are available on request from the corresponding authors.




Publication History

Article published online:
13 June 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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