Transactional models of stress (Lazarus, 1976) emphasize the role of individual factors in moderating the stress response. Numerous researches have focused on the role played by individual characteristics, such as Type A Behaviour, Locus of Control, Hardiness and Self-esteem. Ciarrochi, Deane and Anderson (2002) found that emotional intelligence (e.g. managing others' emotions) significantly moderated the link between stress and mental health. How to enhance emotional intelligence, reduce perceived stress and promote physical and psychological wellbeing simultaneously is another important area of research. A substantial amount of research has shown meditation to be beneficial to both the physiological (e.g. alpha activity maintain and less muscle tension) and psychological well-being (e.g. emotional stability and maturity, decreased depression, positive thinking, increased self-confidence). Meditation not only generates the direct effect of relaxation response for one to balance physical and psychological health state, but also the indirect effect of enhancing his physical and psychological well-being conditions by maintaining positive attitude ward perceived stress. The purpose of this study was to investigate whether meditation experience and emotional intelligence could moderate the relationship between perceived stress and four mental health variables of the GHQ, ie somatic symptoms, anxiety and insomnia, social dysfunction and severe depression. Firstly, to explore the relationship among meditation experience, emotional intelligence, perceived stress and negative mental health. Secondly, to examine the difference in emotional intelligence, perceived stress and negative mental health by the variety of meditation experience. Thirdly, to test whether meditation experience, emotional intelligence (e.g. optimism/mood regulation, appraisal of emotions, social skills and utilization of emotions) and perceived stress could predict significantly the level of negative mental health (e.g. somatic symptoms, anxiety and insomnia, social dysfunction and (or) severe depression). Finally, to investigate whether meditation experience and emotional intelligence could moderate the relationship between perceived stress and negative mental health. A total of 351 working adults (156 females, 195 men, mean age was 36.83 years, SD=8.28) participated in this cross-sectional study and completed a battery of structured questionnaires, which included measures of meditation experience, the Emotional Intelligence Scale (Schutte et al., 1998), the Perceived Stress Scale (Cohen, Kamarck, & Mermelstein, 1983), and the GHQ-28 Inventory (Chan, 1983, 1985; Goldberg & Hillier, 1979; Shek, 1987, 1988). The data obtained were analyzed with Pearson product-moment correlation, Multivariate ANOVA and Hierarchical Regression. The results show that meditation experience, emotional intelligence, perceived stress and negative mental health were significantly correlated. Greater meditation experience was associated with greater emotional intelligence, lower perceived stress, somatic symptoms, anxiety and insomnia. Greater emotional intelligence was associated with lower perceived stress, somatic symptoms, anxiety and insomnia, social dysfunction, and severe depression. Greater perceived stress was associated with greater somatic symptoms, anxiety and insomnia, social dysfunction, and severe depression. In addition, it shows that participators, with over five years of meditation experience exhibited higher emotional intelligence (e.g. appraisal of emotions), less perceived stress and better mental health (e.g. less anxiety and insomnia) than those who had no meditation experience. Moreover, regression analyses reveal that meditation experience, emotional intelligence (e.g. optimism/mood regulation, utilization of emotions) and perceived stress all can predict the level of negative mental health (e.g. somatic symptoms, anxiety and insomnia, social dysfunction and (or) severe depression) closely. However, the utilization of emotions predicted positively the factors of somatic symptoms, anxiety and insomnia, and severe depression. These findings were different from that of some Western research results. These discrepancies may have resulted from the culture difference between the West and the Chinese. Additionally, meditation experience and emotional intelligence (e.g. optimism/mood regulation, appraisal of emotions, social skills) have been found to effectively moderate the relationship between perceived stress and negative mental health (e.g. anxiety and insomnia). In summary, the most significant findings in this study, which not only have supported Ciarrochi, Deane & Anderson's (2002) previous findings, was that emotional intelligence serves as a moderator in the relationship between stress and negative mental health. In addition, it also revealed that meditation experience was also an effective moderating factor.