To know the association between psychological strain and glycosylated hemoglobin (HbA_1c) and to examine roles of smoking and catecholamine excretion as a possible mediator in the association, we measured mood states (Profile of Mood States, POMS), urinary catecholamines (adrenalin, noradrenalin and dopamine) from urine sample in early morning, HbA_1c and fasting plasma glucose (FPG) of 63 male employees on a rest day. After excluding 12 subjects who had diabetes or impaired glucose tolerance or had missing response to the questionnaire, data from 51 subjects were analyzed. The POMS anger-hostility score significantly and positively correlated with HbA_1c (p<0.05), while other scale scores did not (p>0.05). Number of cigarettes smoked per day significantly and positively correlated with HbA_1c (p<0.05). Urinary excretion rate of any catecholamine did not significantly correlate with HbA_1c (p>0.05). Multiple linear regression analysis indicated that the anger-hostility score significantly correlated with HbA_1c, after controlling for number of cigarettes per day (p<0.05). It is suggested that, among mood states, anger-hostility is associated with increased HbA_1c. However, our study failed to find a mediating role of urinary catecholamines or smoking on the association between the mood and HbA_1c.