Cent Eur J Public Health 2013, 21(2):88-91 | DOI: 10.21101/cejph.a3807

The Association between Gene Polymorphisms of Glutathione S-transferase T1/M1 and Type 1 Diabetes in Slovak Children and Adolescents

Jarmila Vojtková1, Peter Ďurdík1, Miriam Čiljaková1, Zuzana Michnová1, Tomáš Turčan1, Eva Babušíková2
1 Department of Pediatrics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin and University Hospital Martin, Martin, Slovakia
2 Department of Medical Biochemistry, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Martin, Slovakia

Background: Considering a dramatic increase in the incidence of type 1 diabetes (T1D) worldwide, current research focuses on complex etiology of T1D where immune system, environmental and genetic factors play a significant role. Glutathione S-transferase family of enzymes protects tissue from oxidative damage which is discussed in the context of T1D. The aim of the study was to investigate an association of glutathione S-transferase mu 1 (GST M1) and glutathione S-transferase theta 1 (GST T1) polymorphisms with type 1 diabetes.

Methods: 163 children, 116 with type 1diabetes and 47 healthy controls, at the age 6-19 years were enrolled to the study. Basic anthropometric, biochemical parameters and GST T1 diabetes and M1 polymorphisms were established in each subject.

Results: Subjects with T1D had significantly lower concentration of uric acid compared to the healthy subjects (212.85±57.10 µmol/l vs. 269.57±72.53; p<0.001). GST T1 null genotype was more frequent in patients with diabetes compared to the healthy controls (36.2% vs. 21.3%) and represented 2.1-fold increased risk of T1D of borderline statistical significance (OR=2.1; 95% CI=0.949-4.648; p=0.06). GST T1 null/M1 wild genotype combination was more frequent in patients with diabetes (25.9% vs. 10.6%) and represented 2.9-fold increased risk for T1D development (OR=2.93; 95% CI=1.061-8.095; p=0.032).

Conclusion: The study indicates that GST T1 null genotype and GST T1 null/M1 wild combination could be considered a risk factor for type 1 diabetes development in Slovak children and adolescents.

Keywords: type 1 diabetes, glutathione S-transferase T1 and M1, gene polymorphisms

Received: June 27, 2012; Revised: January 24, 2013; Accepted: January 24, 2013; Published: June 1, 2013  Show citation

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Vojtková J, Ďurdík P, Čiljaková M, Michnová Z, Turčan T, Babušíková E. The Association between Gene Polymorphisms of Glutathione S-transferase T1/M1 and Type 1 Diabetes in Slovak Children and Adolescents. Cent Eur J Public Health. 2013;21(2):88-91. doi: 10.21101/cejph.a3807. PubMed PMID: 24053064.
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