[Purpose] This study aimed to identify factors that determine the kinematic coupling behavior of calcaneal pronation/supination and shank rotation in a standing position. [Participants and Methods] Study participants included 15 healthy adults (30 legs). Kinematic coupling behavior was quantified as the linear regression coefficient (kinetic chain ratio [KCR]) of the angle of shank rotation against the angle of calcaneal pronation-to-supination measured using a 3-dimensional motion analysis system during pronation and supination of both feet while standing. The relationship between the KCR and the foot bone alignment was also analyzed using 35 parameters that were evaluated based on plain radiography. [Results] Greater the height of the medial longitudinal arch, and greater the backward tilt of the long axis of the talus and the backward tilt of the talar articular surface of the calcaneus, larger the KCR. This alignment differed between the genders. [Conclusion] This study suggested that the KCR increases as the subtalar joint axis approaches the long axis of the shank secondary to the lifting of the medial longitudinal arch of the foot and decreases as the subtalar joint axis approaches the long axis of the foot secondary to the lowering of the medial longitudinal arch of the foot.