A collection of 1814 human chromosome 7-specific STSs.

  1. G G Bouffard,
  2. L M Iyer,
  3. J R Idol,
  4. V V Braden,
  5. A F Cunningham,
  6. L A Weintraub,
  7. R M Mohr-Tidwell,
  8. D C Peluso,
  9. R S Fulton,
  10. M P Leckie, and
  11. E D Green

Abstract

An established goal of the ongoing Human Genome Project is the development and mapping of sequence-tagged sites (STSs) every 100 kb, on average, across all human chromosomes. En route to constructing such a physical map of human chromosome 7, we have generated 1814 chromosome 7-specific STSs. The corresponding PCR assays were designed by the use of DNA sequence determined in our laboratory (79%) or generated elsewhere (21%) and were demonstrated to be suitable for screening yeast artificial chromosome (YAC) libraries. This collection provides the requisite landmarks for constructing a physical map of chromosome 7 at < 100-kb average spacing of STSs.

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  1. doi: 10.1101/gr.7.1.59 Genome Res. 7: 59-64 Copyright © Cold Spring Harbor Laboratory Press

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