Periapical Inflammation after Coronal Microbial Inoculation of Dog Roots Filled with Gutta-Percha or Resilon

doi:10.1097/01.don.0000140569.33867.bf

Journal of Endodontics

Volume 31, Issue 2, February 2005, Pages 91-96

https://doi.org/10.1097/01.don.0000140569.33867.bf Get rights and content

Abstract

A dog model was used to assess and compare in vivo the efficacy of gutta-percha and AH26 sealer versus Resilon with Epiphany primer and sealer [Resilon “Monoblock” System (RMS)] filled roots in preventing apical periodontitis subsequent to coronal inoculation with oral microorganisms. There were 56 vital roots in the premolars of seven adult beagle dogs aseptically instrumented, filled, and temporized. The roots were randomly divided into four experimental groups (Coronal Leakage Model) and one negative control group and filled as follows: group 1—lateral condensation of gutta-percha and AH26 sealer (n=12); group 2—vertical condensation of gutta-percha and AH26 sealer (n=12); group 3—lateral condensation of RMS (n=12); group 4—vertical condensation of RMS (n=10); negative control (n=10)—gutta-percha and AH26 sealer or RMS root fillings using lateral or vertical condensation techniques as in groups 1 to 4. Positive control—57 additional premolar roots were instrumented, infected and not filled (beginning of the Entombment Model experiment). The premolars in groups 1 to 4 were accessed again, inoculated with dental plaque scaled from the dog's teeth, and temporized. This fresh innoculum of microorganisms was repeated on two more occasions at monthly intervals. The teeth in the negative control group were not accessed again and remained undisturbed. On the 14-wk postcoronal inoculation, dogs were euthanized, and jaw blocks prepared for histologic evaluation under a light microcope. Mild inflammation was observed in 82% (18 of 22) of roots filled with gutta-percha and AH26 sealer that was stastistically more than roots filled with RMS (19% or 4 of 21) and roots in the negative control (22% or 2 of 9) (McNemar paired analysis, p < 0.05). The Resilon “Monoblock” System was associated with less apical periodontitis, which may be because of its superior resistance to coronal microleakage.

Section snippets

Materials and Methods

Seven adult beagle dogs were selected for this research. There were 10 premolars used in each dog, half were used for another experimental model whereby the roots were infected, apical periodontitis induced and then filled (Entombment Model). This manuscript addresses the Coronal Leakage Model where vital roots were instrumented, filled immediately, and then challenged by coronally placed oral microorganisms. The study protocol was approved by the University of North Carolina Institutional

Results

All dogs tolerated the operative procedures well throughout the observation period. There was no evidence of swelling or sinus tract associated with any of the treated teeth. One tooth fractured and was excluded from the study (two roots filled with gutta-percha and AH26 sealer). One root filled with RMS using the lateral condensation technique was lost in histological sectioning. The overall results are summarized in Table 1.

Radiographic observations at all time intervals revealed no signs of

Discussion

This in vivo study is a continuation of the evaluation of this new RMS to assess if the superior bacterial leakage resistance found in our in vitro model (¹⁸) is related to apical periodontitis, the disease of interest in endodontics. While the RMS was clearly superior in the in vitro model, that model is unable to determine if the leakage found relates to disease. This study was designed to assess if the apparent superiority of the material can be duplicated under physiologic functional

Acknowledgment

This study was supported in part by the Endodontic Research Grant of the American Association of Endodontists Foundation.

The authors thank Eric Simmons (Department of Oral Microbiology, University of North Carolina, Chapel Hill, NC) for his assistance in culturing the microbiological specimens.

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Citation Excerpt :
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: Address requests for reprints to Dr. Martin Trope, Department of Endodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, 27599; e-mail: [email protected].

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Basic Research-TechnologyPeriapical Inflammation after Coronal Microbial Inoculation of Dog Roots Filled with Gutta-Percha or Resilon

Abstract

Section snippets

Materials and Methods

Results

Discussion

Acknowledgment

Oral Surg Oral Med Oral Pathol

Oral Surg Oral Med Oral Pathol

J Endod

J Endod

Oral Surg Oral Med Oral Pathol

J Endod

J Endod

J Endod

J Endod

J Endod

Basic Research-Technology
Periapical Inflammation after Coronal Microbial Inoculation of Dog Roots Filled with Gutta-Percha or Resilon