Basic Research-TechnologyPeriapical Inflammation after Coronal Microbial Inoculation of Dog Roots Filled with Gutta-Percha or Resilon
Section snippets
Materials and Methods
Seven adult beagle dogs were selected for this research. There were 10 premolars used in each dog, half were used for another experimental model whereby the roots were infected, apical periodontitis induced and then filled (Entombment Model). This manuscript addresses the Coronal Leakage Model where vital roots were instrumented, filled immediately, and then challenged by coronally placed oral microorganisms. The study protocol was approved by the University of North Carolina Institutional
Results
All dogs tolerated the operative procedures well throughout the observation period. There was no evidence of swelling or sinus tract associated with any of the treated teeth. One tooth fractured and was excluded from the study (two roots filled with gutta-percha and AH26 sealer). One root filled with RMS using the lateral condensation technique was lost in histological sectioning. The overall results are summarized in Table 1.
Radiographic observations at all time intervals revealed no signs of
Discussion
This in vivo study is a continuation of the evaluation of this new RMS to assess if the superior bacterial leakage resistance found in our in vitro model (18) is related to apical periodontitis, the disease of interest in endodontics. While the RMS was clearly superior in the in vitro model, that model is unable to determine if the leakage found relates to disease. This study was designed to assess if the apparent superiority of the material can be duplicated under physiologic functional
Acknowledgment
This study was supported in part by the Endodontic Research Grant of the American Association of Endodontists Foundation.
The authors thank Eric Simmons (Department of Oral Microbiology, University of North Carolina, Chapel Hill, NC) for his assistance in culturing the microbiological specimens.
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2014, Acta BiomaterialiaCitation Excerpt :Another issue that has not been considered in most in vivo canine studies is the role played by over-instrumentation or overfilling in the tissue response, in particular between what may be classified as “no inflammation” vs. “mild inflammation”. For example, in the study by Shipper et al. [180], 82% of the dog teeth that were obturated with gutta-percha and an epoxy-resin-based sealer exhibited had mild periapical inflammation and 18% had no inflammation. By contrast, 19% of the dog teeth that were obturated with the polycaprolactone-based root filling material and its accompanying self-etching sealer exhibited mild periapical inflammation and 81% had no inflammation.
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Address requests for reprints to Dr. Martin Trope, Department of Endodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, 27599; e-mail: [email protected].