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Age-Related Decrease in the Inducibility of Heat-Shock Protein 70 in Human Peripheral Blood Mononuclear Cells

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Abstract

We have investigated the effect of age and of the presence of proinflammatory cytokines on Hsp 70 production in human peripheral blood mononuclear cells, using flow cytometry. Twenty-seven women and 23 men, all apparently healthy, participated in the study. At 37°C, the percentage of Hsp 70-producing monocytes and lymphocytes, as well as the level of Hsp 70 in monocytes, were negatively influenced by age. After exposure of the cells to 42°C, the increase of Hsp 70 production was more pronounced in monocytes than in lymphocytes; both the intensity of Hsp 70 production and the percentage of Hsp 70-producing cells were negatively influenced by the age of the subjects, as well for monocytes as for lymphocytes. There was a negative correlation between the intensity of Hsp 70 production by monocytes exposed to 42°C and the serum levels of tumor necrosis factor-α and interleukin-6. In conclusion, in human monocytes and lymphocytes, heat-induced Hsp 70 production is reduced with increasing age and is negatively influenced in monocytes by proinflammatory cytokines.

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  1. Geriatric Unit, Academic Hospital, Free University Brussels (VUB), Belgium

    R. Njemini, M. Vanden Abeele, M. Lambert, S. Vandebosch & T. Mets

  2. HLA and Immunology Laboratory, Academic Hospital, Free University Brussels (VUB), Belgium

    C. Demanet

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  1. R. Njemini
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  2. M. Vanden Abeele
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Njemini, R., Abeele, M.V., Demanet, C. et al. Age-Related Decrease in the Inducibility of Heat-Shock Protein 70 in Human Peripheral Blood Mononuclear Cells. J Clin Immunol 22, 195–205 (2002). https://doi.org/10.1023/A:1016036724386

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