Cortisol levels in children with Attention-Deficit/Hyperactivity Disorder
Introduction
Regulation of the Hypothalamus–Pituitary–Adrenal axis (HPA-axis) and its end product cortisol differs among persons with certain psychiatric disorders when compared with controls (Tsigos and Chrousos, 2002). For instance, depression is associated with high levels of cortisol, possibly as an expression of impaired glucocorticoid-mediated feedback inhibition (Pariante and Lightman, 2008). A hyperactivation of the HPA axis has been reported not only for depression but also for obsessive–compulsive disorder (Gustafsson et al., 2008), panic disorder (Wedekind et al., 2000; Abelson et al., 2007) and anorexia nervosa (Lo Sauro et al., 2008). A decreased functioning of the HPA-axis has been associated with atypical depression (Tsigos and Chrousos, 2002), chronic fatigue syndrome (Roberts et al., 2004; Papadopoulos and Cleare, 2011; Tak et al., 2011), posttraumatic stress disorder (PTSD) (Yehuda, 1998; Heim et al., 2000) and fibromyalgia (Griep et al., 1998; Riva et al., 2010). Several reports concern Attention-Deficit/Hyperactivity Disorder (ADHD), a neurobehavioral developmental disorder with three subgroups characterized by predominantly symptoms of inattention (ADHD-I), hyperactivity/impulsiveness (ADHD-HI) or both types, “combined” (ADHD-C).
However, the results are not conclusive; both hyper- and hypo-functioning have been reported as well as no differences in comparisons with children without ADHD-symptoms (for a short review, see below). Hypothetically, an association between a down-regulated HPA axis and ADHD fits with theories that regard ADHD as a consequence of under-arousal (Fairchild, 2010). One such theory is the optimal stimulation theory (Zentall and Zentall, 1983) which suggests that the manifestations of ADHD can be seen as expressions of increased activity which aims at promoting arousal, e.g. through shifts in attention, talking, seeking stimulation, risk-taking or aggressive behavior (Zentall, 2005).
The secretion of cortisol follows a diurnal cycle, characterized by high levels on awakening, a further increase during the morning and a gradual decrease over the day until midnight (Tsigos and Chrousos, 2002). Abnormalities of the HPA axis in children with ADHD were originally suggested in 1993 by Kaneko et al. who found that a normal diurnal cortisol rhythm (defined as maximum levels in the morning and minimum levels in the evening) was less frequent in children with ADHD than in (adult) controls. They also reported that the children with ADHD less frequently than the controls responded with suppression when exposed to dexamethasone, a synthetic steroid with capacity of suppressing the secretion of ACTH (dexamethasone suppression test [DST]) (Slater et al., 1962). Since then studies of cortisol have focused either on a dysfunctional circadian rhythm or an impaired reactivity after DST or exposure to an experimental stressor. Further, studies have focused on cortisol levels in relation to severity of ADHD symptoms, different diagnostic subtypes or co-morbidity.
Several authors have reported an association between low cortisol levels and either hyperactivity (Kaneko et al., 1993; Blomqvist et al., 2007; Ma et al., 2011) or ADHD symptoms in general (Scerbo and Kolko, 1994; King et al., 1998). Others have reported low cortisol levels in children with co-morbid oppositional defiant disorder (ODD) (van Goozen et al., 1998; Kariyawasam et al., 2002; Freitag et al., 2009) or disruptive behavior (Hastings et al., 2009). In contrast several studies have failed to demonstrate any relation between cortisol levels and ADHD in general (Pesonen et al., 2011; Wang et al., 2011), for attention (Sondeijker et al., 2007), for hyperactivity (Hatzinger et al., 2007) and for ADHD with aggressive behavior (Schulz et al., 1997). Studies on stress reactivity display a similar ambiguity. A blunted response has been associated with ADHD (King et al., 1998; McCarthy et al., 2011), hyperactivity (Hong et al., 2003; van West et al., 2009), inattentive problems (Randazzo et al., 2008; Pesonen et al., 2011) and ODD co-morbidity (Snoek et al., 2004; Hastings et al., 2009). Contrarily, others have not found a decreased cortisol response in children with ADHD in general (Jansen et al., 1999; White and Mulligan, 2005; Christiansen et al., 2010) or with hyperactivity (Hatzinger et al., 2007).
In summary, findings reported on ADHD and cortisol levels are inconclusive. This may reflect methodological limitations such as small samples (only three studies with n > 100), varying – and not rarely vague – diagnostic routines, comparison groups with unknown “contamination” of individuals with ADHD and unsatisfactory control of sampling times. Furthermore, only a few studies have captured the morning cortisol levels by collecting samples when awakening and soon thereafter. Thus, in the present study we aimed at investigating the diurnal levels of saliva cortisol in school aged children (6–17 years of age) with ADHD and age matched healthy comparisons also taking severity, subgroup and co-occurring symptoms into consideration.
Section snippets
Procedure
Children/adolescents (6–17 years of age) of the study group were recruited from four child psychiatry outpatient units in three Swedish counties where the ADHD-diagnosis had been verified by neuropsychiatric assessment. Written project information was presented at a regular examination or – in one county – by mail to the parents and to the child (an age-adapted version). After written informed consent from parents (and child when ≥15 years of age) a questionnaire about current medication
Results
Data on mean cortisol levels on the different sampling occasions and morning increase are presented in Table 3. The diurnal pattern is further described in Fig. 1.
The study group had lower cortisol levels than comparisons at waking up (Mann–Whitney, p < .001), 30 min later (p < .001) and before going to bed (p = .015). No difference was found for the afternoon sample. Similar results were found using the log-scale. The cortisol levels were not influenced by gender, neither in the study group
Discussion
The main findings of this study were that children with ADHD had significantly lower saliva cortisol levels in the morning and evening than non-affected comparisons. Neither gender nor ADHD medication had any influence on cortisol levels. Age however had an influence on cortisol levels; when splitting the group into different age categories only children above 10 years of age had lower levels than healthy comparisons. There was a trend that the morning increase was lower in the study group.
Role of the funding sources
The study was supported by grants from Victoriafonden through the Swedish Brain Foundation (Hjärnfonden) (no specific grant no.). The PhD position of Johan Isaksson was supported by Uppsala University Hospital Research Fund (ALF) (no specific grant no.). The research position of Frank Lindblad was financed by the Swedish Council for Working Life and Social Research, grant no. 2006-0197.
The sponsors of the study had no role in the study design, data collection, data analysis, data
Contributors
Johan Isaksson contributed to the creation of the study design, had responsibility for data collection from the clinical units and for data administration, performed the first statistical analyses, managed the literature searches in collaboration with FL and wrote the first draft of the manuscript – as well as the consecutive revisions – in collaboration with FL.
Kent W Nilsson contributed to the creation of the study design and had the main responsibility for the statistical analyses.
Fred
Conflict of interest
All authors report no biomedical financial interests or potential conflicts of interest.
Acknowledgments
We are grateful to all children who volunteered and to their parents and schools in Uppsala, Enköping and Gävle who made it possible for us to recruit the children. We are also grateful to the child and adolescent psychiatric units in Uppsala, Enköping, Gävle and Falun for contributions to the recruitment procedure. We also want to thank Hans Arinell and Mattias Rehn for statistical assistance and Lars Holmberg for the cortisol analyses.
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