Basic ResearchHistologic Examination of Teeth with Necrotic Pulps and Periapical Lesions Treated with 2 Scaffolds: An Animal Investigation
Section snippets
Methods
All animal experiments were performed in accordance with protocols approved by the Loma Linda University Animal Research Committee. Six 70-day-old male ferrets with immature canine teeth were used in this experiment. Two animals provided the positive controls, and the 4 remaining ferrets provided the experimental teeth. A total of 16 experimental and 8 positive control teeth were used in this study. Preoperative radiographs were obtained to confirm the presence of an open apex and the absence
Results
Clinical evaluation revealed that the teeth in all groups had no evidence of swelling, mobility, or sinus tracts, and all restorations were intact.
In all of the positive control teeth, normal development was noted. The canals were filled with normal pulp tissue lined with odontoblasts subjacent to the predentin (Fig. 1A). Normal physiological dentinal thickening of the root and apical closure were observed along with the presence of multiple apical deltas (normal apex anatomy in ferret teeth).
Discussion
The histologic regeneration of pulp-like tissues has been shown in previous studies 4, 5, 19. Ideally, the ultimate goal of regenerative endodontics is observation of true, functional pulp tissue with peripheral odontoblasts, which has been found in a de novo study (19). This study with the ferret animal model resulted in the development of hard tissue similar to the morphology of bone and cementum at an unpredictable rate. The studies mentioned above were on human subjects, and the de novo
Acknowledgments
The authors thank Dr George Saukel from the Department of Surgical Pathology at the LLU Medical Center for assisting with the photography of histologic specimens. They also thank Dr Udochukwu Oyoyo for help with the statistical analysis of this investigation.
The authors thank the AAE Foundation for partial support of this investigation.
The authors deny any conflicts of interest related to this study.
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