Basic research—biologyThe Substitution of Chlorhexidine for Doxycycline in MTAD: The Antibacterial Efficacy Against a Strain of Enterococcus faecalis
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Materials and Methods
Fifty single-rooted human anterior teeth (excluding mandibular incisors) were used in this study. The teeth had been stored in gauze soaked with water to prevent dehydration before use. Each tooth was radiographed to confirm the presence of a patent canal. An access opening was prepared on the lingual or occlusal surface of each tooth to expose the root canal system. After removal of the pulp with a barbed broach and irrigation with distilled water, each tooth was then sterilized by using an
Results
All samples in the positive control group showed the presence of turbidity in the test tubes, whereas all of the negative control samples remained free of growth. None of the 10 samples in groups A and C showed any growth after 1 week of incubation. Seven out of 10 samples in group B showed turbidity in the test tubes. The results are summarized in Table 1.
There was a statistical difference between the four experimental groups. The irrigants used in groups A and C were significantly more
Discussion
In the present study, the controls showed that the test bacteria remained viable during the experimental period and contamination did not occur during the sampling protocol. Evaluation of the bacteria using standard microbiological techniques verified the existence of a pure culture.
MTAD and MTAD-C (MTAD with the addition of 0.2% CHX) both disinfected all 10 teeth in group A and group C, respectively. In contrast, MCAD completely eliminated E. faecalis from only 3 of 10 teeth in group B. These
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2011, Journal of EndodonticsCitation Excerpt :Drug combinations are increasingly important in combating the spread of antibiotic resistance in pathogens (28). The substitution of doxycycline with chlorhexidine in MTAD does not generate the same antibacterial effect on E. faecalis as MTAD, and the addition of chlorhexidine does not improve the antibacterial effect of MTAD in root canal models infected with E. faecalis (11). The use of 1.3% NaOCl as an initial rinse results in a 30% reduction in the antimicrobial substantivity of MTAD because of the interaction between NaOCl and doxycycline (32).
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