Inflammatory response of the dental pulp to bacterial irritation

doi:10.1016/S0099-2399(81)80122-7

Journal of Endodontics

Volume 7, Issue 3, March 1981, Pages 100-104

https://doi.org/10.1016/S0099-2399(81)80122-7 Get rights and content

The response of the dental pulp to a carious lesion may involve both specific and nonspecific inflammatory reactions. Nonreversible tissue damage as well as repair and healing can result. Information is presented regarding possible mechanisms involved in the development of pulpitis in relation to infection in dentin. Data from animal experiments are presented; both the immediate and late responses of the pulp tissues are studied. These pulpal responses were produced by bacterial and antigenic substances allowed to penetrate through intact but exposed dentin.

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Cited by (101)

Recent advancements in hydrogels as novel tissue engineering scaffolds for dental pulp regeneration
2024, International Journal of Biological Macromolecules
Although conventional root canal treatment offers an effective therapeutic solution, it negatively affects the viability of the affected tooth. In recent years, pulp regeneration technology has emerged as a novel method for treating irreversible pulpitis due to its ability to maintain tooth vitality. The successful implementation of this technique depends on scaffolds and transplantation of exogenous stem cells or recruitment of endogenous stem cells. Accordingly, the three-dimensional structure and viscoelastic characteristics of hydrogel scaffolds, which parallel those of the extracellular matrix, have generated considerable interest. Furthermore, hydrogels support the controlled release of regenerative drugs and to load a wide variety of bioactive molecules. By integrating antibacterial agents into the hydrogel matrix and stimulating an immune response, root canal disinfection can be significantly improved and the rate of pulp regeneration can be accelerated. This review aims to provide an overview of the clinical applications of hydrogels that have been reported in the last 5 years, and offer a comprehensive summary of the different approaches that have been utilized for the optimization of hydrogel scaffolds for pulp regeneration. Advancements and challenges in pulp regeneration using hydrogels treating aged teeth are discussed.
Prostaglandin E<inf>2</inf>-Transporting Pathway and Its Roles via EP2/EP4 in Cultured Human Dental Pulp
2023, Journal of Endodontics
Prostaglandin E₂ (PGE₂) exerts biological actions through its transport pathway involving intracellular synthesis, extracellular transport, and receptor binding. This study aimed to determine the localization of the components of the PGE₂-transporting pathway in human dental pulp and explore the relevance of PGE₂ receptors (EP2/EP4) to angiogenesis and dentinogenesis.
Protein localization of microsomal PGE₂ (mPGES)synthase, PGE₂ transporters (multidrug resistance-associated protein-4 [MRP4] and prostaglandin transporter [PGT]), and EP2/EP4 was analyzed using double immunofluorescence staining. Tooth slices from human third molars were cultured with or without butaprost (EP2 agonist) or rivenprost (EP4 agonist) for 1 week. Morphometric analysis of endothelial cell filopodia was performed to evaluate angiogenesis, and real-time polymerase chain reaction was performed to evaluate angiogenesis and odontoblast differentiation markers.
MRP4 and PGT were colocalized with mPGES and EP2/EP4 in odontoblasts and endothelial cells. Furthermore, MRP4 was colocalized with mPGES and EP4 in human leukocyte antigen-DR-expressing dendritic cells. In the tooth slice culture, EP2/EP4 agonists induced significant increases in the number and length of filopodia and mRNA expression of angiogenesis markers (vascular endothelial growth factor and fibroblast growth factor-2) and odontoblast differentiation markers (dentin sialophosphoprotein and collagen type 1).
PGE₂-producing enzyme (mPGES), transporters (MRP4 and PGT), and PGE₂-specific receptors (EP2/EP4) were immunolocalized in various cellular components of the human dental pulp. EP2/EP4 agonists promoted endothelial cell filopodia generation and upregulated angiogenesis- and odontoblast differentiation-related genes, suggesting that PGE₂ binding to EP2/EP4 is associated with angiogenic and dentinogenic responses.
Injectable decellularized dental pulp matrix-functionalized hydrogel microspheres for endodontic regeneration
2023, Acta Biomaterialia
Citation Excerpt :
When the dental pulp is infected by the bacteria of carious or traumatic origin, inflammation and tissue necrosis occur, eventually leading to loss of functions [1, 2].
The sufficient imitation of tissue structures and components represents an effective and promising approach for tissue engineering and regenerative medicine applications. Dental pulp disease is one of the most common oral diseases, although functional pulp regeneration remains challenging. Herein, we propose a strategy that employs hydrogel microspheres incorporated with decellularized dental pulp matrix-derived bioactive factors to simulate a pulp-specific three-dimensional (3D) microenvironment. The dental pulp microenvironment-specific microspheres constructed by this regenerative strategy exhibited favorable plasticity, biocompatibility, and biological performances. Human dental pulp stem cells (hDPSCs) cultured on the constructed microspheres exhibited enhanced pulp-formation ability in vitro. Furthermore, the hDPSCs-microcarriers achieved the regeneration of pulp-like tissue and new dentin in a semi-orthotopic model in vivo. Mechanistically, the decellularized pulp matrix-derived bioactive factors mediated the multi-directional differentiation of hDPSCs to regenerate the pulp tissue by eliciting the secretion of crucial bioactive cues. Our findings demonstrated that a 3D dental pulp-specific microenvironment facilitated by hydrogel microspheres and dental pulp-specific bioactive factors regenerated the pulp-dentin complex and could be served as a promising treatment option for dental pulp disease.
Injectable bioscaffolds are increasingly used for regenerative endodontic treatment. Despite their success related to their ability to load stem cells, bioactive factors, and injectability, conventional bulk bioscaffolds have drawbacks such as ischemic necrosis in the central region. Various studies have shown that ischemic necrosis in the central region can be corrected by injectable hydrogel microspheres. Unfortunately, pristine microspheres or microspheres without dental pulp-specific bioactive factor would oftentimes fail to regulate stem cells fates in dental pulp multi-directional differentiation. Our present study reported the biofabrication of dental pulp-derived decellularized matrix functionalized gelatin microspheres, which contained dental pulp-specific bioactive factors and have the potential application in endodontic regeneration.
The effect of dentin surface treatment with disinfectant on the shear bonding strength of luting cements
2022, Journal of Dental Sciences
Few studies have comprehensively assessed the shear bonding strength of the luting cements between abutments and fixed partial dentures after dentin surface treatment with disinfectants. The purpose of the study was to evaluate the effect of three commonly used disinfectants (2.5% sodium hypochlorite, 0.2% chlorhexidine, and 0.2% benzalkonium chloride) on the shear bonding strength of four luting cements.
Teeth were mounted on Teflon cylinders and prepared for dentin exposure. Three different disinfectants were used to treat the dentin surface. Nickel-chromium posts were cemented with resin cement, glass ionomer cement, polycarboxylate cement, or zinc phosphate cement. The shear bonding strength of the cement was examined using an Instron testing machine. Two-way analysis of variance (ANOVA) was used to examine the differences in shear bonding strength between the cements. If a statistically significant difference was found through ANOVA, a post hoc test with Tukey's honest significant difference was conducted.
Disinfectants significantly decreased the shear bonding strength of resin cement, with 2.5% sodium hypochlorite causing the most substantial decrease. The zinc phosphate cement group displayed minimal shear bonding strength regardless of the disinfectant used.
The presence of 2.5% sodium hypochlorite significantly reduced the shear bonding strength of resin cements. During permanent cementation of indirect restorations, the choice of luting cement paired with the proper disinfectant is of utmost importance to maintain the shear bonding strength.
Expression Profiling of S100 Proteins in Healthy and Irreversibly Inflamed Human Dental Pulps
2022, Journal of Endodontics
Several S100 proteins have been shown to play an important role in the innate immune response to infection and in regenerative processes. However, they have scarcely been investigated during inflammation of the dental pulp. Therefore, in this study, we performed gene expression profiling of S100 proteins in healthy and inflamed human dental pulps.
Tissue samples of human dental pulps were used, including 15 clinically diagnosed as symptomatic irreversible pulpitis (SIP), 7 as asymptomatic irreversible pulpitis (AIP), and 19 as healthy pulp (HP). S100 gene expression levels were quantitatively evaluated for S100A1, -A2, -A3, -A4, -A6, -A7, -A8, -A9, -A10, -A11, -A13, -A14, and -A16 by the quantitative polymerase chain reaction technique. In order to monitor the status of inflammation and degradation of pulp tissues, IL-8, COX-2, and HMGB-1 gene expression was also analyzed with GAPDH serving as the reference gene. Differential expression rates for each target gene between SIP, AIP, and HP were evaluated by analysis of variance followed by the Bonferroni post hoc test.
Significantly reduced gene expression levels could be detected in SIP compared with HP for S100A1, -A2, -A3, -A4, -A6, -A10, and -A13 and for HMGB-1, whereas the gene expression of S100A8 and -A14 and IL-8 were significantly increased. In AIP, significantly increased expression levels compared with HP were only detected for S100A14 and -A16 and IL-8, with other genes of interest not being altered.
The present study revealed significant differences in gene expression profiles of S100 proteins comparing samples from healthy and inflamed dental pulp. More pronounced differences were observed for symptomatic than for asymptomatic pulpitis.
Characterization of Root Canal Microbiota in Teeth Diagnosed with Irreversible Pulpitis
2021, Journal of Endodontics
Previous studies have shown that in teeth presenting with symptoms of irreversible pulpitis (IP), bacteria and their by-products driving inflammation are confined mainly within the coronal pulpal tissue. The present study aimed to determine the presence and identity of bacteria within pulps presenting with clinical symptoms of IP using molecular methods.
Samples were obtained from 30 adult patients presenting to the dental emergency department with signs and symptoms of IP. After meticulous surface decontamination, the pulp space was accessed, and clinical samples were collected from inflamed pulp tissue using sterile paper points. Genomic DNA was extracted from the clinical samples, and quantification of bacteria was performed using quantitative polymerase chain reaction targeting the conserved 16S ribosomal RNA (rRNA) gene. To characterize the microbial composition, the V3–V5 hypervariable regions of the 16S rRNA gene were amplified and subjected to next-generation sequencing on the MiSeq platform (Illumina, San Diego, CA).
Of the 30 teeth that presented with IP, half of the intracanal samples had a substantial bacterial load (16S rRNA copies) within the IP vital pulp as determined by quantitative polymerase chain reaction. Next-generation sequencing microbial identification was successful in 7 intracanal samples and yielded 187 bacterial operational taxonomic units within the IP samples. The most abundant genera observed among the vital cases were Veillonella (16%), Streptococcus (13%), Corynebacterium (10%), Cutibacterium (9.3%), and Porphyromonas (5.7%).
The current study highlighted the evidence of vital teeth diagnosed as IP harboring considerable bacterial loads and composed of genera reflective of established endodontic pathology and thus may offer insights into the initial events preceding pulpal necrosis.

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¹: Dr. Bergenholtz is on the faculty of odontology, University of Goteborg, Sweden, and is currently visiting associate professor at the Department of Endodontics, The University of Michigan, Ann Arbor, Michigan 48109.

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Inflammatory response of the dental pulp to bacterial irritation

Oral Surg

Arch Oral Biol

Arch Oral Biol

Arch Oral Biol

Oral Surg

Oral Surg

Caries profunda

Norske Tarnnlaegeforen Tid

Healing processes in the pulp on capping

Tissue changes in the dental pulp

Odontol Tidskr

Pulpal response to early dental caries

J Dent Res

Indirect capping and the treatment of deep carious lesions

Int Dent J

Dental pulp conditions in relation to carious lesions

Int Dent J