Research paper
Estimation of volume fractions in nervous tissue with an image analyzer

https://doi.org/10.1016/0165-0270(82)90014-0Get rights and content

Abstract

The calculation of volume fractions in nervous tissue is a method often used in neuroanatomy. In ontogenetic studies and problems concerning aging, as well as after experimental procedures, volume fractions are calculated in order to quantify the results. Grey level index (GLI) and grey cell coefficient (GCC) are different parameters correlated to the volume density of cellular elements in nervous tissue. The grey cell coefficient (GCC) is defined as the volume fraction of cellular elements in a griseum (grey matter area) and is estimated by a basic stereological method (point counting) from observations in two dimensions. The grey level index (GLI), measured with an automatic image analyzer, is the areal proportion of projected profiles of all stained elements within a volume given by the area of the measuring field and the thickness of section. GLI and GCC are compared in specimens obtained by a special histological procedure, and a method to estimate volume fractions by means of GLI-measurement is described.

References (44)

  • H. Adhami

    Die photometrische Bestimmung des Cortexzell- und Graugehalts auf der Grundlage der Nissl-Bildes

    Acta Anat.

    (1973)
  • N.H. Bass et al.

    Quantitative cytoarchitectonic distribution of neurons, glia and DNA in rat cerebral cortex

    J. comp. Neurol.

    (1971)
  • H.S. Bennett et al.

    Science and art in preparing tissue embedded in plastic for light microscopy, with special reference to glucol methacrylate, glass knives and simple stains

    Stain Technol.

    (1976)
  • S.M. Blinkov

    Die Glia

  • E. Braak

    A method of firmly attaching 4–10 μm Araldite serial sections to glass slides for light microscopic staining procedures

    Stain Technol.

    (1977)
  • S.O. Brattgard

    Microscopical determination of the thickness of histological sections

    J. roy. Microsc. Soc.

    (1954)
  • H.W. Chalkley

    Method for quantitative morphologic analysis of tissue

    J. nat. Cancer Inst.

    (1943)
  • L. Einarson

    A method for progressive selective staining of Nissl- and nuclear-substances in nerve cells

    Amer. J. Pathol.

    (1932)
  • K. Fleischhauer et al.

    Cell densities in the various layers of the rabbit's striate area

    Anat. Embryol.

    (1979)
  • K. Fleischhauer et al.

    A revised cytoarchitectonic map of the neocortex of the rabbit (Oryctolagus cuniculus)

    Anat. Embryol.

    (1980)
  • A.D. Hally

    A counting method for measuring the volumes of tissue components in microscopical sections

    Quart. J. microsc. Sci.

    (1964)
  • H. Haug

    Die Treffertmethode, ein Verfahren zur quantitativen Analyse im histologischen Schnitt

    Z. Anat. Entwickl.-Gesch.

    (1955)
  • H. Haug

    Remarks on the determination and significance of the gray cell coefficient

    J. comp. Neurol.

    (1956)
  • H. Haug

    Quantitative Untersuchungen an der Sehrinde

    (1958)
  • H. Haug

    Bedeutung und Grenzen der quantitativen messmethoden in der Hisotlogie

  • A. Henning

    Diskussion der Fehler bei der Volumbestimmung kugeliger Körper oder Hohlräume aus den Schnittprojektionen

    Z. wiss. Mikrosk.

    (1957)
  • D. Heumann et al.

    Postnatal development of the mouse cerebral cortex. IV. Evolution of the total cortical volume, of the population of neurons and glial cells

    J. Hirnforsch.

    (1978)
  • A. Holmes

    Petrographic Methods and Calculations

    (1927)
  • K. John

    Über die Konstanz der Schnittdicke beim Schneiden mit dem Mikrotom

    Z. wiss. Mikrosk.

    (1929)
  • C. Kraus

    Veränderungen der Paraffinschnitte durch das Mikrotomieren und das nachfolgende Aufziehen

    J. Hirnforsch.

    (1962)
  • G. Leuba et al.

    Postnatal development of the mouse cerebral cortex. I. Quantitative cytoarchitectonics of some motor and sensory areas

    J. Hirnforsch.

    (1977)
  • J.I. Nurnberger

    Direct enumeration of cells of the brain

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