Changes in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis

doi:10.1016/0012-1606(88)90225-4

Developmental Biology

Volume 125, Issue 2, February 1988, Pages 441-450

https://doi.org/10.1016/0012-1606(88)90225-4 Get rights and content

Abstract

We have previously characterized monoclonal antibodies against chick brain cells. One of them (14-2B2) brightly stained all capillaries in frozen sections of chick brain. Here we show that this antibody is directed against chick fibronectin. Using this antibody and polyclonal antibodies against laminin, we have studied the development of the vascular extracellular matrix. Vasculogenesis, the development of capillaries from in situ differentiating endothelial cells, was studied in yolk sac blood islands and intraembryonic dorsal aorta. Blood islands produced high levels of fibronectin but not laminin. Early intraembryonic capillaries all expressed fibronectin but little if any laminin. The dorsal aorta of a 6-day-old chick embryo has several layers of fibronectin-producing cells, but is devoid of laminin. Laminin expression commenced at Day 8 and by Day 10 an adult-like distribution was found in the aortic vascular wall. Angiogenesis, the formation of capillaries from preexisting vessels, was studied during brain development. Capillary sprouts invading the neuroectoderm at Embryonic Day 4 migrated in a fibronectin-rich matrix devoid of laminin. Ultrastructural immunolocalization demonstrated the presence of fibronectin exclusively on the abluminal site of the endothelial cells. Beginning on Day 6, laminin codistributed with fibronectin in brain capillaries. We conclude that immature capillaries migrate and proliferate in a fibronectin-rich extracellular matrix, which is subsequently remodeled acquiring basement membrane-like characteristics. We suggest that laminin expression is an early indication of vascular maturation.

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^☆: Supported by Basic Research Grant 1–979 from the March of Dimes and National Eye Institute Grant EY05285 to V.L.

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Full paperChanges in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis☆

Abstract

Brain Res

Cell Differ

Cell Differ

Differentiation

Dev. Brain Res

Dev. Biol

Cell

Trends Biochem. Sci

Dev. Biol

Trends Biochem. Sci

J. Biol. Chem

Heavy metal intensification of DAB-based HRP reaction product

J. Histochem. Cytochem

The vascular system of the cerebral cortex

Adv. Anat. Embryol. Cell Biol

Antibodies to fibronectin prevent gastrulation but do not perturb neurulation in gastrulated amphibian embryos

Nature (London)

Biological active synthetic peptides as probes of embryonic development: A competitive peptide inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest cell migration in avian embryo

J. Cell Biol

Chemotaxis of aortic endothelial cells in response to fibronectin

Cancer Res

Alterations in neural crest migration by a monoclonal antibody that affects cell adhesion

J. Cell Biol

Coupled expression and colocalization of 140K cell adhesion molecules, fibronectin, and laminin during morphogenesis and cytodifferentiation of chick lung cells

J. Cell Biol

Blood vessel fibronectin increases in conjunction with endothelial cell proliferation and capillary ingrowth during wound healing

J. Invest. Dermatol

Observations on healing tissue: A combined light and electron microscopic investigation

Philos. Trans. R. Soc. London Ser. B

Vascular endothelium as the vulnerable element in tumours

Acta Radiol. Oncol

Changes in the patterns of collagens and fibronectin during limb-bud chondrogenesis

J. Embryol. Exp. Morphol

Cell adhesion and migration in the early vertebrate embryo: Location and possible role of the putative fibronectin receptor complex

J. Cell Biol

On the development of the aortae, cardinal and umbilical veins, and the other blood vessels of vertebrate embryos from capillaries

Anat. Rec

The development of the vascular pattern within the walls of the central nervous system of the chick embryo

J. Morphol

Angiogenesis

Endothelial cell proliferation during angiogenesis

In vitro modulation by basement membrane components

Lab. Invest

Editorial

Critical factors controlling angiogenesis: Cell products, cell matrix and growth factors

Lab. Invest

Full paper
Changes in the vascular extracellular matrix during embryonic vasculogenesis and angiogenesis☆