Elsevier

Brain Research

Volume 169, Issue 1, 15 June 1979, Pages 139-153
Brain Research

Manipulations of dietary tryptophan: Effects on mouse killing and brain serotonin in the rat

https://doi.org/10.1016/0006-8993(79)90380-9Get rights and content

Abstract

Maintaining rats on a tryptophan-free diet for 4–6 days induced mouse killing in non-killer rats, and significantly facilitated killing in killer rats, as indicated by shorter latencies to kill the mice. The killing responses were similar in topography to the natural killing responses. These changes in killing behavior did not appear to be due to generalized changes in irritability. The increased killing after maintenance on a tryptophan-free diet was accompanied by a 26% reduction in brain serotonin (5-HT) and a 29% reduction in brain 5-hydroxyindoleacetic acid (5-HIAA). When the tryptophan-free diet was supplemented withl-tryptophan (0.5 or 2%), brain 5-HT and 5-HIAA were increased above control levels, and the rat's killing response appeared normal both in terms of latency and topography, similar to that seen in control chow fed animals. While rats consumed less of the tryptophan-free and tryptophan supplemented diets, control subjects deprived of chow such that they lost as much weight as rats fed the tryptophan-free diet, did not show changes in killing behavior. These results are consistent with the hypothesis that central serotonergic systems exert inhibitory control over mouse killing behavior in rats.

Reference (68)

  • GrantL.D. et al.

    Muricide after serotonin depleting lesions of midbrain raphe nuclei

    Pharmacol. Biochem. Behav.

    (1973)
  • JacobsB.L. et al.

    Effect of indolealkylamine manipulations on locomotor activity in rats

    Neuropharmacology

    (1974)
  • KulkarniA.S.

    Muricidal block produced by 5-hydroxytryptophan and various drugs

    Life Sci.

    (1968)
  • MalickJ.B.

    Effects of age and food deprivation on the development of muricidal behavior in rats

    Physiol. Behav.

    (1975)
  • McLainW.C. et al.

    Central catechol-and indoleamine systems and aggression

    Pharmacol. Biochem. Behav.

    (1974)
  • MiczekK.A. et al.

    Para-chlorophenylalanine, serotonin and killing behavior

    Pharmacol. Biochem. Behav.

    (1975)
  • PaxinosG. et al.

    5-Hydroxytryptamine depletion with para-chlorophenylalanine: effects on eating, drinking, irritability, muricide, and copulation

    Pharmacol. Biochem. Behav.

    (1977)
  • PolskyR.H.

    Hunger, prey feeding, and predatory aggression

    Behav. Biol.

    (1975)
  • SheardM.H.

    The effect of p-chlorophenylalanine on behavior in rats: relation to brain serotonin and 5-hydroxyindoleacetic acid

    Brain Research

    (1969)
  • SrebroB. et al.

    Behavioral effects of selective midbrain raphe lesions in the rat

    Brain Research

    (1975)
  • ThurmondJ.B. et al.

    Effects of dietary tyrosine, phenylalanine, and tryptophan on aggression in mice

    Pharmacol. Biochem. Behav.

    (1977)
  • VergnesM.

    Déclenchement de réaction d'agression interspécifique après lésion amygdalienne chez le rat

    Physiol. Behav.

    (1975)
  • VergnesM. et al.

    Controˆle inhibiteur du comportement d'agression interspécifique sur rat: Système sérotoninergique du raphéet afférences olfactives

    Brain Research

    (1974)
  • VergnesM. et al.

    Lésions du raphéet réaction d'aggression interspécifique rat-souris. Effects comportementaux et biochimiques

    Brain Research

    (1973)
  • WeissmanA.

    Behavioral pharmacology of p-chlorophenylalanine (PCPA)

  • WoodworthC.H.

    Attack elicited in rats by electrical stimulation of the lateral hypothalamus

    Physiol. Behav.

    (1971)
  • WurtmanR.J. et al.

    Control of brain neurotransmitter synthesis by precursor availability and nutritional state

    Biochem. Pharmacol.

    (1976)
  • AndenN.E. et al.

    Ascending monoamine neurons to the telencephalon and diencephalon

    Acta physiol. scand.

    (1966)
  • AvisH.H. et al.

    Mediation of rat-mouse interspecific aggression by cage odor

    Psychon. Sci.

    (1971)
  • BandlerR.J.

    Facilitation of aggressive behaviour in rat by direct cholinergic stimulation of the hypothalamus

    Nature (Lond.)

    (1969)
  • BandlerR.J. et al.

    Animals spontaneously attacked by rats

    Commun. Behav. Biol.

    (1970)
  • BarrG.A. et al.

    Neuropharmacological regulation of mouse killing by rats

    Behav. Biol.

    (1976)
  • BlundellJ.E.

    Is there a role for serotonin (5-hydroxytryptamine) in feeding?

    Int. J. Obesity

    (1977)
  • BowersD.

    Facilitative Effects of Electric Shock on Mouse Killing by Hooded Rats

    (1974)
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