Abstract
There are many studies on the effects of meditation interventions and some about meditation retreats in a group of participants, but how to study the brain of a single subject who decides to seclude himself/herself in a retreat? The aim of this study was to present a proof of concept for the feasibility of a single-subject analysis, which allows the study of meditation effects on the brain. We tested the proposed approach in a meditator entering a 5.5-week retreat. Neuroimaging data were acquired using diffusion tensor imaging, structural magnetic resonance imaging, and functional magnetic resonance imaging under resting-state condition. Comparing pre vs. post data, we noticed reductions in the visual cortices (primary and secondary), Brodmann area 8, and anterior cingulate cortex, while the amplitude of low-frequency fluctuations increased in the dorsolateral prefrontal cortex. A greater regional homogeneity was observed in the precuneus before the retreat. Reductions in the anterior cingulate cortex may be due to the retreat environment and routine and its focus on the management of mental affliction, allowing participants to develop a focused and calm mind (lessened conflicts). Other changes may be related to both less mind wandering and more recruitment of the attentional circuitry during the meditation practices.
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References
Brefczynski-Lewis, J. A., Lutz, A., Schaefer, H. S., Levinson, D. B., & Davidson, R. J. (2007). Neural correlates of attentional expertise in long-term meditation practitioners. Proceedings of the National Academy of Sciences U S A, 104(27), 11483–11488.
Brewer, J. A., Worhunsky, P. D., Gray, J. R., Tang, Y. Y., Weber, J., & Kober, H. (2011). Meditation experience is associated with differences in default mode network activity and connectivity. Proceedings of the National Academy of Sciences U S A, 2011, 108(50), 20254–20259.
Chao-Gan, Y., & Yu-Feng, Z. (2010). DPARSF: A MATLAB toolbox for “pipeline” data analysis of resting-state fMRI. Frontiers in System Neuroscience, 4, 13.
Ekman, P. (2003). Emotions revealed: recognizing faces and feelings to improve communication and emotional life. New York: Henry holt and Company, LLC.
Elliott, J. C., Wallace, B. A., & Giesbrecht, B. (2014). A week-long meditation retreat decouples behavioral measures of the alerting and executive attention networks. Frontiers in Human Neuroscience, 8, 69.
Farb, N. A., Segal, Z. V., Mayberg, H., Bean, J., McKeon, D., Fatima, Z., & Anderson, A. K. (2007). Attending to the present: mindfulness meditation reveals distinct neural modes of self-reference. Social, Cognitive and Affective Neuroscience, 2(4), 313–322.
Fischl, B., & Dale, A. M. (2000). Measuring the thickness of the human cerebral cortex from magnetic resonance images. Proceedings of the National Academy of Sciences U S A, 97(20), 11050–11055.
Fransson, P., & Marrelec, G. (2008). The precuneus/posterior cingulate cortex plays a pivotal role in the default mode network: evidence from a partial correlation network analysis. NeuroImage, 42(3), 1178–1184.
Hagler Jr., D. J., Saygin, A. P., & Sereno, M. I. (2006). Smoothing and cluster thresholding for cortical surface-based group analysis of fMRI data. NeuroImage, 33(4), 1093–1103.
Hayasaka, S., & Nichols, T. E. (2003). Validating cluster size inference: random field and permutation methods. NeuroImage, 20(4), 2343–2356.
Jenkinson, M., Beckmann, C. F., Behrens, T. E., Woolrich, M. W., & Smith, S. M. (2012). FSL. NeuroImage, 62(2), 782–790.
Kemeny, M. E., Foltz, C., Cavanagh, J. F., Cullen, M., Giese-Davis, J., Jennings, P., Rosenberg, E. L., Gillath, O., Shaver, P. R., Wallace, B. A., & Ekman, P. (2012). Contemplative/emotion training reduces negative emotional behavior and promotes prosocial responses. Emotion, 12(2), 338–350.
Kozasa, E. H., Radvany, J., Barreiros, M. A., Leite, J. R., & Amaro Jr., E. (2008). Preliminary functional magnetic resonance imaging Stroop task results before and after a Zen meditation retreat. Psychiatry and Clinical Neuroscience, 62(3), 366.
Lazar, S. W., Kerr, C. E., Wasserman, R. H., Gray, J. R., Greve, D. N., Treadway, M. T., McGarvey, M., Quinn, B. T., Dusek, J. A., Benson, H., Rauch, S. L., Moore, C. I., & Fischl, B. (2005). Meditation experience is associated with increased cortical thickness. Neuroreport, 16(17), 1893–1897.
Luders, E., Phillips, O. R., Clark, K., Kurth, F., Toga, A. W., & Narr, K. L. (2012). Bridging the hemispheres in meditation: thicker callosal regions and enhanced fractional anisotropy (FA) in long-term practitioners. NeuroImage, 61(1), 181–187.
MacLean, K. A., Ferrer, E., Aichele, S. R., Bridwell, D. A., Zanesco, A. P., Jacobs, T. L., King, B. G., Rosenberg, E. L., Sahdra, B. K., Shaver, P. R., Wallace, B. A., Mangun, G. R., & Saron, C. D. (2010). Intensive meditation training improves perceptual discrimination and sustained attention. Psychological Science, 21(6), 829–839.
Merabet, L. B., Hamilton, R., Schlaug, G., Swisher, J. D., Kiriakopoulos, E. T., Pitskel, N. B., Kauffman, T., & Pascual-Leone, A. (2008). Rapid and reversible recruitment of early visual cortex for touch. PloS One, 3(8), e3046.
Raichle, M. E., MacLeod, A. M., Snyder, A. Z., Powers, W. J., Gusnard, D. A., & Shulman, G. L. (2001). A default mode of brain function. Proceedings of the National Academy of Sciences U S A, 98(2), 676–682.
Rosenberg, E. L., Zanesco, A. P., King, B. G., Aichele, S. R., Jacobs, T. L., Bridwell, D. A., MacLean, K. A., Shaver, P. R., Ferrer, E., Sahdra, B. K., Lavy, S., Wallace, B. A., & Saron, C. D. (2015). Intensive meditation training influences emotional responses to suffering. Emotion, 15(6), 775–790.
Sahdra, B. K., MacLean, K. A., Ferrer, E., Shaver, P. R., Rosenberg, E. L., Jacobs, T. L., Zanesco, A. P., King, B. G., Aichele, S. R., Bridwell, D. A., Mangun, G. R., Lavy, S., Wallace, B. A., & Saron, C. D. (2011). Enhanced response inhibition during intensive meditation training predicts improvements in self-reported adaptive socioemotional functioning. Emotion, 11(2), 299–312.
Segal, Z. V., Kennedy, S., Gemar, M., Hood, K., Pedersen, R., & Buis, T. (2006). Cognitive reactivity to sad mood provocation and the prediction of depressive relapse. Archives in General Psychiatry, 63(7), 749–755.
Sheline, Y. I., Barch, D. M., Price, J. L., Rundle, M. M., Vaishnavi, S. N., Snyder, A. Z., Mintun, M. A., Wang, S., Coalson, R. S., & Raichle, M. E. (2009). The default mode network and self-referential processes in depression. Proceedings of the National Academy of Sciences of the United States of America, 106(6), 1942–1947.
Sliz, D., Smith, A., Wiebking, C., Northoff, G., & Hayley, S. (2012). Neural correlates of a single-session massage treatment. Brain Imaging & Behavior, 6(1), 77–87.
Smith, S. M., Jenkinson, M., Johansen-Berg, H., Rueckert, D., Nichols, T. E., Mackay, C. E., Watkins, K. E., Ciccarelli, O., Cader, M. Z., Matthews, P. M., & Behrens, T. E. (2006). Tract-based spatial statistics: voxelwise analysis of multi-subject diffusion data. NeuroImage, 31(4), 1487–1505.
Song, X. W., Dong, Z. Y., Long, X. Y., Li, S. F., Zuo, X. N., Zhu, C. Z., He, Y., Yan, C. G., & Zang, Y. F. (2011). REST: a toolkit for resting-state functional magnetic resonance imaging data processing. PloS One, 6(9), e25031.
Szekely, A., Silton, R. L., Heller, W., Miller, G. A., & Mohanty, A. (2016). Differential functional connectivity of rostral anterior cingulate cortex during emotional interference. Social Cognitive Affecivet Neuroscence, 12(3), 476–486 pii: nsw137.
Tang YY, Hölzel BK, Posner MI. The neuroscience of mindfulness meditation. Nat Rev Neurosci. 2015;16(4):213-25.
Tori, C. D., & Emavardhana, T. (1997). Changes in self-concept, ego defense mechanism, and religiosity following seven-day Vipassana meditation retreats. Journal for the Scientific Study of Religion, 36(2), 194–206.
Volz, K. G., Schubotz, R. I., & von Cramon, D. Y. (2004). Why am I unsure? Internal and external attributions of uncertainty dissociated by fMRI. NeuroImage, 21(3), 848–857.
Volz, K. G., Schubotz, R. I., & von Cramon, D. Y. (2005). Variants of uncertainty in decision-making and their neural correlates. Brain Research Bulletin, 67(5), 403–412.
Wallace, B. A. (2007). Genuine happiness: Meditation as the path to fulfillment. Hoboken: Wiley.
Wang, H. Y., Xu, G. Q., Ni, M. F., Zhang, C. H., Sun, X. P., Chang, Y., & Zhang, B. W. (2017). Neural mechanisms of implicit cognitive reappraisal: preceding descriptions alter emotional response to unpleasant images. Neuroscience, 347, 65–75.
Zang, Y., Jiang, T., Lu, Y., He, Y., & Tian, L. (2004). Regional homogeneity approach to fMRI data analysis. NeuroImage, 22(1), 394–400.
Zang, Y. F., He, Y., Zhu, C. Z., Cao, Q. J., Sui, M. Q., Liang, M., Tian, L. X., Jiang, T. Z., & Wang, Y. F. (2007). Altered baseline brain activity in children with ADHD revealed by resting-state functional MRI. Brain Development, 29(2), 83–91.
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We would like to thank the Instituto Israelita de Ensino e Pesquisa Albert Einstein for the financial support and Adriano de Santis for MRI data collection.
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The second author of this paper is the participant in the study, and the third author was the meditation instructor of the retreat.
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Sato, J.R., Kozasa, E.H., Wallace, B.A. et al. Neuroimaging Data From a Single Participant Before and After a Meditation Retreat: a Proof of Concept Study. J Cogn Enhanc 1, 235–241 (2017). https://doi.org/10.1007/s41465-017-0025-4
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DOI: https://doi.org/10.1007/s41465-017-0025-4