The salivary protein statherin is an inhibitor of spontaneous and secondary precipitation of hydroxyapatite (HAp). It is also detected in enamel pellicle. The N-terminal region of statherin is involved in its adsorption onto tooth surfaces, and, calcium binding. A peptide (StN21) was designed with a 21 amino acid sequence identical to the N-terminus of statherin. The aim was to measure the effect of StN21 on the rate of mineral loss in a model system for dental caries and erosion using HAp subjected to artificial carious and erosive conditions. StN21 was synthesised using Fmoc chemistry. A surface of each HAp block was exposed to solution containing StN21 at concentrations 9.4–376 μmol L−1 (in phosphate buffer) for 24 h. Controls were HAp exposed to buffer only, and HAp exposed to lysozyme. Demineralising solution (0.1 mol L−1 acetic acid, pH 4.5, 1.0 mmol L−1 calcium and 0.6 mmol L−1 phosphate) was circulated past the HAp blocks at 0.4 mL min-1 to mimic carious and erosive conditions. Scanning microradiography was used to measure the rate of mineral loss for demineralisation periods of 3 weeks. The rate of mineral loss of the samples exposed to StN21 was reduced by ∼40% compared to the controls, but no dependence on the concentration of StN21 was observed at the concentrations used. StN21 has been shown to be a potent and stable peptide that has potential as a preventive/therapeutic agent in the treatment of enamel erosion and dental caries.
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Abbreviations
- XMT:
-
X-ray microtomography
- HBTU:
-
[2-(1H-benzotriazol-1yl)-1,1,3,3-tetramethyl-uronium hexafluorophosphate] HOBt (N-hydroxybenzotriazol)
- MBTE:
-
methyl-tertiary butylether
- TFA:
-
trifluoracetic acid
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JK gratefully acknowledges financial support through a Wellcome VIP award.
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Kosoric, J., Williams, R.A.D., Hector, M.P. et al. A Synthetic Peptide Based on a Natural Salivary Protein Reduces Demineralisation in Model Systems for Dental Caries and Erosion. Int J Pept Res Ther 13, 497–503 (2007). https://doi.org/10.1007/s10989-007-9085-0
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DOI: https://doi.org/10.1007/s10989-007-9085-0