Skip to main content

Advertisement

SpringerLink
Log in

Periodontal regeneration in experimentally-induced alveolar bone dehiscence by an improved porous biphasic calcium phosphate ceramic in beagle dogs

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Regeneration of lost periodontium is the focus of periodontal therapy. To achieve the effective regeneration, a number of bone graft substitute materials have been developed. This study aimed to investigate the histological response in alveolar bone dehiscences which were filled with an improved biphasic calcium phosphate (BCP) ceramic with more reasonable pore diameter, pore wall thickness and porosity. Twenty-four alveolar bone dehiscences were made surgically in twelve beagle dogs by reflecting mucoperiosteal flaps on the buccal aspect of bilateral lower second premolars and removing alveolar bone. The left dehiscences were treated with BCP ceramic and the contralaterals were cured with the open flap debridement (OFD) as controls. Three dogs were used at week 4, 12, and 24 respectively. Histological observations were processed through three-dimensional micro-computed tomographic imaging, fluorescence and light microscopy. The histological study indicated that the biphasic ceramic was biocompatible, and regeneration was achieved more effectively through the BCP treatment. There were also arrest of epithelial migration apically and formation of new bone and cementum, as well as proliferation of fibrous connective tissues that became attached to the newly formed cementum at week 24, while there was no significant periodontal regeneration in the OFD group only with epithelial tissue migrating into the dehiscence regions. Clinically speaking, though the surgical location formed a limitation to the application of the improved BCP on the periodontal regeneration, the actual result was positive. It proved that the BCP had biocompatibility and was able to act as a stable scaffold to induce periodontal regeneration effectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

BCP:

Biphasic calcium phosphate

μCT:

Micro-computed tomography

CaP:

Calcium phosphate

TCP:

Tricalcium phosphate

β-TCP:

β-Tricalcium phosphate

HA:

Hydroxyapatite

PDL:

Periodontal ligament

OFD:

Open flap debridement

References

  1. S. Nyman, J. Lindhe, T. Karring, H. Rylander, J. Clin. Periodontol. 9, 290 (1982). doi:10.1111/j.1600-051X.1982.tb02095.x

    Article  CAS  Google Scholar 

  2. J. Gottlow, S. Nyman, J. Lindhe et al., J. Clin. Periodontol. 13, 604 (1986). doi:10.1111/j.1600-051X.1986.tb00854.x

    Article  CAS  Google Scholar 

  3. M.A. Brunsvold, J.T. Mellonig, Periodontology 2000 1, 80 (1993). doi:10.1111/j.1600-0757.1993.tb00209.x

  4. J. Murakami, T. Kato, S. Kawai, S. Akiyama, A. Amano, I. Morisaki, J. Periodontol. 79, 721 (2008). doi:10.1902/jop.2008.070400

    Article  CAS  Google Scholar 

  5. D.L. Cochran, J.M. Wozney, Periodontology 2000 19, 40 (1999). doi:10.1111/j.1600-0757.1999.tb00146.x

  6. T. Karring, J. Lindhe, P. Cortellini, Clinical Periodontology and Implant Dentistry (Munksgaard, Copenhagen, 2003)

    Google Scholar 

  7. M. Jarcho, Clin. Orthop. Relat. Res. J. 157, 259 (1981)

    CAS  Google Scholar 

  8. F.H. Albee, Ann. Surg. 71, 32 (1920). doi:10.1097/00000658-192001000-00006

    Article  CAS  Google Scholar 

  9. N. Passuti, G. Daculsi, J.M. Rogez, S. Martin, J.V. Bainvel, Clin. Orthop. Relat. Res. 248, 169 (1989)

    Google Scholar 

  10. A.O. Ransford, T. Morley, M.A. Edgar et al., J. Bone Joint Surg. Br. 80, 13 (1998). doi:10.1302/0301-620X.80B1.7276

    Article  CAS  Google Scholar 

  11. R. Cavagna, G. Daculsi, J.M. Bouler, J. Long Term Eff. Med. Implant 9, 403 (1999)

    CAS  Google Scholar 

  12. J. Delecrin, S. Takahashi, F. Gouin, N. Passuti, Spine 25, 563 (2000). doi:10.1097/00007632-200003010-00006

    Article  CAS  Google Scholar 

  13. K. de Groot, Biomaterials 1, 47 (1980). doi:10.1016/0142-9612(80)90059-9

    Article  Google Scholar 

  14. L.L. Hench, J. Wilson, Science 226, 630 (1984). doi:10.1126/science.6093253

    Article  CAS  Google Scholar 

  15. C.J. Damien, J.R. Parsons, J. Appl. Biomater. 2, 187 (1991). doi:10.1002/jab.770020307

    Article  CAS  Google Scholar 

  16. K. de Groot, R. Geesink, C.P. Klein, P. Serekian, J. Biomed. Mater. Res. 21, 1375 (1987). doi:10.1002/jbm.820211203

    Article  Google Scholar 

  17. A.A. Mirtchi, J. Lemaitre, N. Terao, Biomaterials 10, 475 (1989). doi:10.1016/0142-9612(89)90089-6

    Article  CAS  Google Scholar 

  18. P. Ducheyne, J.M. Cuckler, Clin. Orthop. Relat. Res. 276, 102 (1992)

    Google Scholar 

  19. K.A. Hing, S.M. Best, W. Bonfield, J. Mater. Sci. Mater. Med. 10, 135 (1999). doi:10.1023/A:1008929305897

    Article  CAS  Google Scholar 

  20. J.F. De Oliveira, P.F. De Aguiar, A.M. Rossi et al., Artif. Organs 27, 406 (2003). doi:10.1046/j.1525-1594.2003.07247.x

    Article  Google Scholar 

  21. T.J. Webster, C. Ergun, R.H. Doremus, R.W. Siegel, R. Bizios, Biomaterials 22, 1327 (2001). doi:10.1016/S0142-9612(00)00285-4

    Article  CAS  Google Scholar 

  22. H.J. Erli, M. Ruger, C. Ragoss et al., Biomaterials 27, 1270 (2006). doi:10.1016/j.biomaterials.2005.08.001

    Article  CAS  Google Scholar 

  23. R. Cancedda, A. Cedola, A. Giuliani et al., Biomaterials 28, 2505 (2007). doi:10.1016/j.biomaterials.2007.01.022

    Article  CAS  Google Scholar 

  24. R. Muller, P. Ruegsegger, Stud. Health Technol. Inform. 40, 61 (1997)

    CAS  Google Scholar 

  25. T. Hildebrand, A. Laib, R. Muller et al., J. Bone Miner. Res. 14, 1167 (1999). doi:10.1359/jbmr.1999.14.7.1167

    Article  CAS  Google Scholar 

  26. O. Gauthier, R. Muller, D. von Stechow et al., Biomaterials 26, 5444 (2005). doi:10.1016/j.biomaterials.2005.01.072

    Article  CAS  Google Scholar 

  27. U. Sakallioglu, G. Acikgoz, B. Ayas et al., Biomaterials 25, 1831 (2004). doi:10.1016/S0142-9612(03)00468-X

    Article  CAS  Google Scholar 

  28. S. Garrett, G. Bogle, Periodontology 2000 1, 100 (1993). doi:10.1111/j.1600-0757.1993.tb00211.x

  29. J. Caton, S. Nyman, H. Zander, J. Clin. Periodontol. 7, 224 (1980). doi:10.1111/j.1600-051X.1980.tb01965.x

    Article  CAS  Google Scholar 

  30. C.S. Kim, S.H. Choi, J.K. Chai et al., J. Periodontol. 75, 229 (2004). doi:10.1902/jop.2004.75.2.229

    Article  CAS  Google Scholar 

  31. C.S. Kim, S.H. Choi, K.S. Cho et al., J. Clin. Periodontol. 32, 583 (2005). doi:10.1111/j.1600-051X.2005.00729.x

    Article  Google Scholar 

  32. Y.J. Yeo, D.W. Jeon, C.S. Kim et al., J. Biomed. Mater. Res. B Appl. Biomater. 72, 86 (2005). doi:10.1002/jbm.b.30121

    Article  Google Scholar 

  33. S.N. Bhaskar, J.M. Brady, L. Getter et al., Oral. Surg. Oral. Med. Oral. Pathol. 32, 336 (1971). doi:10.1016/0030-4220(71)90238-6

    Article  CAS  Google Scholar 

  34. P. Weiss, P. Layrolle, L.P. Clergeau et al., Biomaterials 28, 3295 (2007). doi:10.1016/j.biomaterials.2007.04.006

    Article  CAS  Google Scholar 

  35. G. Daculsi, R.Z. LeGeros, C. Deudon, Osseo-coalescence versus osseo-integration. Scanning Microscopy 4, 309 (1990)

    CAS  Google Scholar 

  36. H. Yuan, Z. Yang, J.D. De Bruij, K. De Groot, X. Zhang, Biomaterials 22, 2617 (2001). doi:10.1016/S0142-9612(00)00450-6

    Article  CAS  Google Scholar 

  37. L.L. Hench, Science 208, 826 (1980). doi:10.1126/science.6246576

    Article  CAS  Google Scholar 

  38. N.M. Farina, F.M. Guzon, M.L. Pena, A.G. Cantalapiedra, J. Mater. Sci. Mater. Med. 19, 1565 (2008). doi:10.1007/s10856-008-3400-y

    Google Scholar 

  39. H. Yuan, Z. Yang, Y. Li, X. Zhang, J.D. De Bruijn, K. De Groot, J. Mater. Sci. Mater. Med. 9, 723 (1998). doi:10.1023/A:1008950902047

    Article  CAS  Google Scholar 

  40. H. Yuan, K. Kurashina, J.D. de Bruijn, Y. Li, K. de Groot, X. Zhang, Biomaterials 20, 1799 (1999). doi:10.1016/S0142-9612(99)00075-7

    Article  CAS  Google Scholar 

  41. H. Yuan, J.D. De Bruijn, Y. Li, J. Feng, Z. Yang, K. De Groot et al., J. Mater. Sci. Mater. Med. 12, 7 (2001). doi:10.1023/A:1026792615665

    Article  CAS  Google Scholar 

  42. U. Ripamonti, Biomaterials 17, 31 (1996). doi:10.1016/0142-9612(96)80752-6

    Article  CAS  Google Scholar 

  43. N. Kawai, S. Niwa, M. Sato, Y. Sato, Y. Suwa, I. Ichihara, J. Biomed. Mater. Res. 37, 1 (1997). doi:10.1002/(SICI)1097-4636(199710)37:1<1::AID-JBM1>3.0.CO;2-W

    Article  CAS  Google Scholar 

  44. E. Tsuruga, H. Takita, H. Itoh, Y. Wakisaka, Y. Kyboki, J. Biochem. 121, 317 (1997)

    Google Scholar 

  45. D. Olton, J. Li, M.E. Wilson, T. Rogers, J. Close, L. Huang et al., Biomaterials 28, 1267 (2007). doi:10.1016/j.biomaterials.2006.10.026

    Article  CAS  Google Scholar 

  46. M.A.W. Merkx, J.C. Matthias, M. Hans-Peter, Biomaterials 20, 2029 (1999). doi:10.1016/S0142-9612(99)00105-2

    Article  CAS  Google Scholar 

  47. U.M. Wikesjo, P. Guglielmoni, A. Promsudthi et al., J. Clin. Periodontol. 26, 392 (1999). doi:10.1034/j.1600-051X.1999.260610.x

    Google Scholar 

  48. H.Y. Kim, C.S. Kim, G.J. Jhon et al., J. Peridontol. 73, 1457 (2002). doi:10.1902/jop.2002.73.12.1457

    Google Scholar 

  49. J.S. Park, S.H. Choi, I.S. Moon et al., J. Clin. Peridontol. 30, 443 (2003). doi:10.1034/j.1600-051X.2003.10283.x

  50. S.H. Choi, C.K. Kim, K.S. Cho et al., J. Peridontol. 73, 63 (2002)

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People’s Republic of China

    Han Shi, Ning Zhao, Yangxi Chen & Yunmao Liao

  2. Department of Prosthodontics School of Stomatology, The Fourth Military Medical University, Xi’an, People’s Republic of China

    Jia Ma

Authors
  1. Han Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jia Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ning Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yangxi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yunmao Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yangxi Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shi, H., Ma, J., Zhao, N. et al. Periodontal regeneration in experimentally-induced alveolar bone dehiscence by an improved porous biphasic calcium phosphate ceramic in beagle dogs. J Mater Sci: Mater Med 19, 3515–3524 (2008). https://doi.org/10.1007/s10856-008-3524-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-008-3524-0

Keywords

Search

Navigation