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Gepubliceerd in: Journal of Autism and Developmental Disorders 4/2011

01-04-2011 | Original Paper

Non-Invasive Evaluation of the GABAergic/Glutamatergic System in Autistic Patients Observed by MEGA-Editing Proton MR Spectroscopy Using a Clinical 3 Tesla Instrument

Auteurs: Masafumi Harada, Masako M. Taki, Ayumi Nose, Hitoshi Kubo, Kenji Mori, Hiromu Nishitani, Tsuyoshi Matsuda

Gepubliceerd in: Journal of Autism and Developmental Disorders | Uitgave 4/2011

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Abstract

Amino acids related to neurotransmitters and the GABAergic/glutamatergic system were measured using a 3 T-MRI instrument in 12 patients with autism and 10 normal controls. All measurements were performed in the frontal lobe (FL) and lenticular nuclei (LN) using a conventional sequence for n-acetyl aspartate (NAA) and glutamate (Glu), and the MEGA-editing method for GABA. The GABA level and [GABA]/[NAA] ratio were significantly lower (p < 0.01) in the FL, but not the LN, in patients with autism compared to normal controls. The [GABA]/[Glu] ratio in the FL was also significantly lower (p < 0.05) in the patients than in the normal controls, thus suggesting a possible abnormality in the regulation between GABA and Glu.
Literatuur
go back to reference Aasly, J., Silfvenius, H., Aas, T. C., Sonnewald, U., Olivecrona, M., Juul, R., et al. (1999). Proton magnetic resonance spectroscopy of brain biopsies from patients with intractable epilepsy. Epilepsy Research, 35, 211–217.PubMedCrossRef Aasly, J., Silfvenius, H., Aas, T. C., Sonnewald, U., Olivecrona, M., Juul, R., et al. (1999). Proton magnetic resonance spectroscopy of brain biopsies from patients with intractable epilepsy. Epilepsy Research, 35, 211–217.PubMedCrossRef
go back to reference Aldred, S., Moore, K. M., Fitzgerald, M., & Waring, R. H. (2003). Plasma amino acid levels in children with autism and their families. Journal of Autism and Developmental Disorders, 33, 449–454.CrossRef Aldred, S., Moore, K. M., Fitzgerald, M., & Waring, R. H. (2003). Plasma amino acid levels in children with autism and their families. Journal of Autism and Developmental Disorders, 33, 449–454.CrossRef
go back to reference Baslow, M. H. (2003). N-acetylaspartate in the vertebrate brain: Metabolism and function. Neurochemical Research, 28, 941–953.PubMedCrossRef Baslow, M. H. (2003). N-acetylaspartate in the vertebrate brain: Metabolism and function. Neurochemical Research, 28, 941–953.PubMedCrossRef
go back to reference Bauman, M., & Kemper, T. L. (1985). Histoanatomic observations of the brain in early infantile autism. Neurology, 35, 866–874.PubMed Bauman, M., & Kemper, T. L. (1985). Histoanatomic observations of the brain in early infantile autism. Neurology, 35, 866–874.PubMed
go back to reference Choi, C., Bhardwaj, P. P., Kalra, S., Casault, C. A., Yasmin, U. S., Allen, P. S., et al. (2007). Measurement of GABA and contaminants in gray and white matter in human brain in vivo. Magnetic Resonance in Medicine, 58, 27–33.PubMedCrossRef Choi, C., Bhardwaj, P. P., Kalra, S., Casault, C. A., Yasmin, U. S., Allen, P. S., et al. (2007). Measurement of GABA and contaminants in gray and white matter in human brain in vivo. Magnetic Resonance in Medicine, 58, 27–33.PubMedCrossRef
go back to reference Choi, I.-Y., Lee, S.-P., Merkle, H., & Shen, J. (2006). In vivo detection of gray and white matter differences in GABA concentration in the human brain. NeuroImage, 33, 85–93.PubMedCrossRef Choi, I.-Y., Lee, S.-P., Merkle, H., & Shen, J. (2006). In vivo detection of gray and white matter differences in GABA concentration in the human brain. NeuroImage, 33, 85–93.PubMedCrossRef
go back to reference Cook, E. H., Courchesne, R. Y., Cox, N. J., Lord, C., Gonen, D., Guter, S. J., et al. (1998). Linkage-disequilibrium mapping of autistic disorder, with 15q11–13 Markers. American Journal of Human Genetics, 62, 1077–1083.PubMedCrossRef Cook, E. H., Courchesne, R. Y., Cox, N. J., Lord, C., Gonen, D., Guter, S. J., et al. (1998). Linkage-disequilibrium mapping of autistic disorder, with 15q11–13 Markers. American Journal of Human Genetics, 62, 1077–1083.PubMedCrossRef
go back to reference Cote, C. J., & Wilson, S. (2006). Guidelines for monitoring and management of pediatric patients during and after sedation for diagnostic and therapeutic procedures: An update. Pediatrics, 118, 2587–2602.PubMedCrossRef Cote, C. J., & Wilson, S. (2006). Guidelines for monitoring and management of pediatric patients during and after sedation for diagnostic and therapeutic procedures: An update. Pediatrics, 118, 2587–2602.PubMedCrossRef
go back to reference Devito, T. J., Drost, D. J., Neufeld, R. W., Rajakumar, N., Pavlosky, W., Williamson, P., et al. (2007). Evidence for cortical dysfunction in autism: A proton magnetic resonance spectroscopic imaging study. Biological Psychiatry, 61, 465–473.PubMedCrossRef Devito, T. J., Drost, D. J., Neufeld, R. W., Rajakumar, N., Pavlosky, W., Williamson, P., et al. (2007). Evidence for cortical dysfunction in autism: A proton magnetic resonance spectroscopic imaging study. Biological Psychiatry, 61, 465–473.PubMedCrossRef
go back to reference Fatemi, S. H., Folsom, T. D., Reutiman, T. J., Thuras, P. D. (2008). Expression of GABAB receptors is altered in brains of subjects with autism. Cerebellum Nov 12 (Epub ahead of print). Fatemi, S. H., Folsom, T. D., Reutiman, T. J., Thuras, P. D. (2008). Expression of GABAB receptors is altered in brains of subjects with autism. Cerebellum Nov 12 (Epub ahead of print).
go back to reference Fatemi, S. H., Halt, A. R., Stary, J. M., Kanodia, R., Schulz, S. C., & Realmuto, G. R. (2002). Glutamic acid decarboxylase 65 and 67 kDa proteins are reduced in autistic parietal and cerebellar cortices. Biological Psychiatry, 52, 805–810.PubMedCrossRef Fatemi, S. H., Halt, A. R., Stary, J. M., Kanodia, R., Schulz, S. C., & Realmuto, G. R. (2002). Glutamic acid decarboxylase 65 and 67 kDa proteins are reduced in autistic parietal and cerebellar cortices. Biological Psychiatry, 52, 805–810.PubMedCrossRef
go back to reference Fatemi, S. H., Reutiman, T. J., Folsom, T. D., & Thuras, P. D. (2009). GABAA receptor downregulation in brains of subjects with autism. Journal of Autism and Developmental Disorders, 39, 223–230.PubMedCrossRef Fatemi, S. H., Reutiman, T. J., Folsom, T. D., & Thuras, P. D. (2009). GABAA receptor downregulation in brains of subjects with autism. Journal of Autism and Developmental Disorders, 39, 223–230.PubMedCrossRef
go back to reference Friedman, S. D., Shaw, D. W. W., Artru, A. A., Dawson, G., Petropoulos, H., & Dager, S. R. (2006). Gray and white matter brain chemistry in young children with autism. Archives of General Psychiatry, 63, 786–794.PubMedCrossRef Friedman, S. D., Shaw, D. W. W., Artru, A. A., Dawson, G., Petropoulos, H., & Dager, S. R. (2006). Gray and white matter brain chemistry in young children with autism. Archives of General Psychiatry, 63, 786–794.PubMedCrossRef
go back to reference Friedman, S. D., Shaw, D. W., Artru, A. A., Richards, T. L., Gardner, J., Dawson, G., et al. (2003). Regional brain chemical alterations in young children with autism spectrum disorder. Neurology, 60, 100–107.PubMed Friedman, S. D., Shaw, D. W., Artru, A. A., Richards, T. L., Gardner, J., Dawson, G., et al. (2003). Regional brain chemical alterations in young children with autism spectrum disorder. Neurology, 60, 100–107.PubMed
go back to reference Guptill, J. T., Booker, A. B., Gibbs, T. T., Kemper, T. L., Bauman, M. L., & Blatt, G. J. (2007). [3H]-FLunitrazepam-labeled benzodiazepine binding sites in the hippocampal formation in autism: A multiple concentration autoradiographic study. Journal of Autism and Developmental Disorders, 37, 911–920.PubMedCrossRef Guptill, J. T., Booker, A. B., Gibbs, T. T., Kemper, T. L., Bauman, M. L., & Blatt, G. J. (2007). [3H]-FLunitrazepam-labeled benzodiazepine binding sites in the hippocampal formation in autism: A multiple concentration autoradiographic study. Journal of Autism and Developmental Disorders, 37, 911–920.PubMedCrossRef
go back to reference Harada, M., Kubo, H., Nose, A., Nishitani, H., Matsuda, T. (in press) Measurement of variation in the human cerebral GABA level by in vivo MEGA-editing proton MR spectroscopy using a clinical 3 Tesla instrument and its dependence on brain region and the female menstrual cycle. Hum Brain Mapp. Harada, M., Kubo, H., Nose, A., Nishitani, H., Matsuda, T. (in press) Measurement of variation in the human cerebral GABA level by in vivo MEGA-editing proton MR spectroscopy using a clinical 3 Tesla instrument and its dependence on brain region and the female menstrual cycle. Hum Brain Mapp.
go back to reference Harada, M., Miyoshi, H., Uno, M., Okada, T., Hisaoka, S., Hori, A., et al. (1999). Neuronal impairment of adult moyamoya disease detected by quantified proton MRS and comparison with cerebral perfusion by SPECT with Tc-99m HM-PAO: A trial of clinical quantification of metabolites. Journal of Magnetic Resonance Imaging, 10, 124–129.PubMedCrossRef Harada, M., Miyoshi, H., Uno, M., Okada, T., Hisaoka, S., Hori, A., et al. (1999). Neuronal impairment of adult moyamoya disease detected by quantified proton MRS and comparison with cerebral perfusion by SPECT with Tc-99m HM-PAO: A trial of clinical quantification of metabolites. Journal of Magnetic Resonance Imaging, 10, 124–129.PubMedCrossRef
go back to reference Hisaoka, S., Harada, M., Nishitani, H., & Mori, K. (2001). Regional magnetic resonance spectroscopy of the brain in autistic individuals. Neuroradiology, 43, 496–498.PubMedCrossRef Hisaoka, S., Harada, M., Nishitani, H., & Mori, K. (2001). Regional magnetic resonance spectroscopy of the brain in autistic individuals. Neuroradiology, 43, 496–498.PubMedCrossRef
go back to reference Jensen, J. E., de Frederick, B., & Renshaw, P. F. (2005). Gray and white matter GABA level difference in the human brain using two-dimensional J-resolved spectroscopic imaging. NMR Biomed, 18, 570–576.PubMedCrossRef Jensen, J. E., de Frederick, B., & Renshaw, P. F. (2005). Gray and white matter GABA level difference in the human brain using two-dimensional J-resolved spectroscopic imaging. NMR Biomed, 18, 570–576.PubMedCrossRef
go back to reference Lam, K. S. L., Aman, M. G., & Arnold, L. E. (2006). Neurochemical correlates of autistic disorder: A review of the literature. Research in Developmental Disabilities, 27, 254–289.PubMedCrossRef Lam, K. S. L., Aman, M. G., & Arnold, L. E. (2006). Neurochemical correlates of autistic disorder: A review of the literature. Research in Developmental Disabilities, 27, 254–289.PubMedCrossRef
go back to reference Levitt, J. G., O’Neill, J., McCracken, J. T., Guthrie, D., Toga, A. W., & Alger, J. R. (2003). Proton magnetic resonance spectroscopic imaging in childhood autism. Biological Psychiatry, 54, 1355–1366.PubMedCrossRef Levitt, J. G., O’Neill, J., McCracken, J. T., Guthrie, D., Toga, A. W., & Alger, J. R. (2003). Proton magnetic resonance spectroscopic imaging in childhood autism. Biological Psychiatry, 54, 1355–1366.PubMedCrossRef
go back to reference Lotspeich, L. J., & Ciaranello, R. D. (1993). The neurobiology and genetics of infantile autism. International Review of Neurobiology, 35, 87–129.PubMedCrossRef Lotspeich, L. J., & Ciaranello, R. D. (1993). The neurobiology and genetics of infantile autism. International Review of Neurobiology, 35, 87–129.PubMedCrossRef
go back to reference Ma, D. Q., Whitehead, P. L., Menold, M. M., etMartin, E. R., Ashley-Koch, A. E., Mei, H., et al. (2005). Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism. Am J Hum Genet, 77, 377–388.PubMedCrossRef Ma, D. Q., Whitehead, P. L., Menold, M. M., etMartin, E. R., Ashley-Koch, A. E., Mei, H., et al. (2005). Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism. Am J Hum Genet, 77, 377–388.PubMedCrossRef
go back to reference Meshcer, M., Merkle, H., Kirsch, J., Garwood, M., & Gruetter, R. (1998). Simultaneous in vivo spectral editing and water suppression. NMR in Biomedicine, 11, 266–272.CrossRef Meshcer, M., Merkle, H., Kirsch, J., Garwood, M., & Gruetter, R. (1998). Simultaneous in vivo spectral editing and water suppression. NMR in Biomedicine, 11, 266–272.CrossRef
go back to reference Murphy, D. G., Critchley, H. D., Schmitz, N., MacAlonan, G., van Amelsvoort, T., Robertson, D., et al. (2002). Asperger syndrome: A proton magnetic resonance study of brain. Archives of General Psychiatry, 59, 885–891.PubMedCrossRef Murphy, D. G., Critchley, H. D., Schmitz, N., MacAlonan, G., van Amelsvoort, T., Robertson, D., et al. (2002). Asperger syndrome: A proton magnetic resonance study of brain. Archives of General Psychiatry, 59, 885–891.PubMedCrossRef
go back to reference Novotny, E. J., Jr., Hyder, F., Shevell, M., & Rothman, D. L. (1999). GABA changes with vigabatrin in the developing human brain. Epilepsia., 40, 462–466.PubMedCrossRef Novotny, E. J., Jr., Hyder, F., Shevell, M., & Rothman, D. L. (1999). GABA changes with vigabatrin in the developing human brain. Epilepsia., 40, 462–466.PubMedCrossRef
go back to reference Otsuka, H., Harada, M., Mori, K., Hisaoka, S., & Nishitani, H. (1999). Brain metabolites in the hippocampus-amygdala region and cerebellum in autism: An 1H-MR spectroscopy study. Neuroradiology, 41, 517–519.PubMedCrossRef Otsuka, H., Harada, M., Mori, K., Hisaoka, S., & Nishitani, H. (1999). Brain metabolites in the hippocampus-amygdala region and cerebellum in autism: An 1H-MR spectroscopy study. Neuroradiology, 41, 517–519.PubMedCrossRef
go back to reference Oz, G., Terpstra, M., Tkac, I., Aia, P., Lowary, J., Tuite, P. J., et al. (2006). Proton MRS of the unilateral substantia nigra in the human brain at 4 Tesla: Detection of high GABA concentrations. Magnetic Resonance in Medicine, 55, 296–301.PubMedCrossRef Oz, G., Terpstra, M., Tkac, I., Aia, P., Lowary, J., Tuite, P. J., et al. (2006). Proton MRS of the unilateral substantia nigra in the human brain at 4 Tesla: Detection of high GABA concentrations. Magnetic Resonance in Medicine, 55, 296–301.PubMedCrossRef
go back to reference Page, L. A., Daly, E., Schmitz, N., Simmons, A., Toal, F., Deeley, Q., et al. (2006). In vivo 1H-magnetic resonance spectroscopy study of amygdale-hippocampal and parietal regions in autism. American Journal of Psychiatry, 163, 2189–2192.PubMedCrossRef Page, L. A., Daly, E., Schmitz, N., Simmons, A., Toal, F., Deeley, Q., et al. (2006). In vivo 1H-magnetic resonance spectroscopy study of amygdale-hippocampal and parietal regions in autism. American Journal of Psychiatry, 163, 2189–2192.PubMedCrossRef
go back to reference Petroff, O. A. C., Behar, K. L., Mattson, R. H., & Rothman, D. L. (1996). Human brain γ-aminobutyric acid levels and seizure control following initiation of vigabatrin therapy. Journal of Neurochemistry, 67, 2399–2404.PubMedCrossRef Petroff, O. A. C., Behar, K. L., Mattson, R. H., & Rothman, D. L. (1996). Human brain γ-aminobutyric acid levels and seizure control following initiation of vigabatrin therapy. Journal of Neurochemistry, 67, 2399–2404.PubMedCrossRef
go back to reference Petroff, O. A. C., Ogino, T., & Alger, J. R. (1988). High-resolution proton magnetic resonance spectroscopy of rabbit brain: Regional metabolite levels and postmortem changes. Journal of Neurochemistry, 51, 163–171.PubMedCrossRef Petroff, O. A. C., Ogino, T., & Alger, J. R. (1988). High-resolution proton magnetic resonance spectroscopy of rabbit brain: Regional metabolite levels and postmortem changes. Journal of Neurochemistry, 51, 163–171.PubMedCrossRef
go back to reference Petroff, O. A. C., Rothman, D. L., Behar, K. L., & Mattson, R. H. (1995). Initial observations on effect of vigabatrin on in vivo 1H spectroscopic measurements of γ-aminobutyric acid, glutamate, and glutamine in human brain. Epilepsia, 36, 457–464.PubMedCrossRef Petroff, O. A. C., Rothman, D. L., Behar, K. L., & Mattson, R. H. (1995). Initial observations on effect of vigabatrin on in vivo 1H spectroscopic measurements of γ-aminobutyric acid, glutamate, and glutamine in human brain. Epilepsia, 36, 457–464.PubMedCrossRef
go back to reference Pouwels, P. J. W., Brockmann, K., Kruse, B., Wilken, B., Wick, M., Hanefeld, F., et al. (1999). Regional age dependence of human brain metabolites from infancy to adulthood as detected by quantitative localized proton MRS. Pediatric Research, 46, 474–485.PubMedCrossRef Pouwels, P. J. W., Brockmann, K., Kruse, B., Wilken, B., Wick, M., Hanefeld, F., et al. (1999). Regional age dependence of human brain metabolites from infancy to adulthood as detected by quantitative localized proton MRS. Pediatric Research, 46, 474–485.PubMedCrossRef
go back to reference Provencher, S. E. (1993). Estimation of metabolite concentrations from localized in vivo proton NMR spectra. Magnetic Resonance in Medicine, 30, 672–679.PubMedCrossRef Provencher, S. E. (1993). Estimation of metabolite concentrations from localized in vivo proton NMR spectra. Magnetic Resonance in Medicine, 30, 672–679.PubMedCrossRef
go back to reference Samaco, R. C., Hogart, A., & Lasalle, J. M. (2004). Epigenetic overlap in autism-spectrum neurodevelopmental disorders: MECP2 deficiency causes reduced expression of UBE3A and GABRB3. Human Molecular Genetics, 14, 483–492.PubMedCrossRef Samaco, R. C., Hogart, A., & Lasalle, J. M. (2004). Epigenetic overlap in autism-spectrum neurodevelopmental disorders: MECP2 deficiency causes reduced expression of UBE3A and GABRB3. Human Molecular Genetics, 14, 483–492.PubMedCrossRef
go back to reference Shinohe, A., Hashimoto, K., Nakamura Tsujii, M., Iwata, Y., Tsuchiya, K. J., Sekine, Y., et al. (2006). Increase serum levels of glutamate in adult patients with autism. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 30, 1472–1477.PubMedCrossRef Shinohe, A., Hashimoto, K., Nakamura Tsujii, M., Iwata, Y., Tsuchiya, K. J., Sekine, Y., et al. (2006). Increase serum levels of glutamate in adult patients with autism. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 30, 1472–1477.PubMedCrossRef
go back to reference Terpstra, M., Henry, P.-G., & Gruetter, R. (2003). Measurement of reduced glutathione (GSH) in human brain using LCModel analysis of difference-edited spectra. Magnetic Resonance in Medicine, 50, 19–23.PubMedCrossRef Terpstra, M., Henry, P.-G., & Gruetter, R. (2003). Measurement of reduced glutathione (GSH) in human brain using LCModel analysis of difference-edited spectra. Magnetic Resonance in Medicine, 50, 19–23.PubMedCrossRef
go back to reference Terpstra, M., Ugurbil, K., & Gruetter, R. (2002). Direct in vivo measurement of human cerebral GABA concentration using MEGA-editing at 7 Tesla. Magnetic Resonance in Medicine, 47, 1009–1012.PubMedCrossRef Terpstra, M., Ugurbil, K., & Gruetter, R. (2002). Direct in vivo measurement of human cerebral GABA concentration using MEGA-editing at 7 Tesla. Magnetic Resonance in Medicine, 47, 1009–1012.PubMedCrossRef
go back to reference Weber, O. M., Verhagen, A., Duc, C. O., Meier, D., Leenders, K. L., & Boesiger, P. (1999). Effects of vigabatrin intake on brain GABA activity as monitored by spectrally edited magnetic resonance spectroscopy and positron emission tomography. Magnetic Resonance Imaging, 17, 417–425.PubMedCrossRef Weber, O. M., Verhagen, A., Duc, C. O., Meier, D., Leenders, K. L., & Boesiger, P. (1999). Effects of vigabatrin intake on brain GABA activity as monitored by spectrally edited magnetic resonance spectroscopy and positron emission tomography. Magnetic Resonance Imaging, 17, 417–425.PubMedCrossRef
Metagegevens
Titel
Non-Invasive Evaluation of the GABAergic/Glutamatergic System in Autistic Patients Observed by MEGA-Editing Proton MR Spectroscopy Using a Clinical 3 Tesla Instrument
Auteurs
Masafumi Harada
Masako M. Taki
Ayumi Nose
Hitoshi Kubo
Kenji Mori
Hiromu Nishitani
Tsuyoshi Matsuda
Publicatiedatum
01-04-2011
Uitgeverij
Springer US
Gepubliceerd in
Journal of Autism and Developmental Disorders / Uitgave 4/2011
Print ISSN: 0162-3257
Elektronisch ISSN: 1573-3432
DOI
https://doi.org/10.1007/s10803-010-1065-0

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