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Schedule-induced Polydipsia in Lines of Rats Selectively Bred for High and Low Ethanol Preference

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Abstract

Ethanol drinking was assessed in the P/NP, HAD1/LAD1, and HAD2/LAD2 lines of rats under environmental conditions that produce schedule-induced polydipsia. Female rats (n = 8/line), maintained at 85% of free-feeding body weights, underwent daily 1-h sessions during which 45-mg food pellets were delivered every 60 s. Water, 2, 4, 8, 16, or 32% w/v ethanol solution was available from a single bottle for 8 consecutive sessions at each concentration, with blood-ethanol levels (BELs) determined after selected sessions. P and HAD2 rats drank more water and ethanol than their non-preferring counterparts, while HAD1 and LAD1 rats did not differ. Ethanol intake and BELs were positively correlated (r = 0.75) across lines. Finally, rats were allowed 14 daily choice sessions with 8% ethanol and water concurrently available. Water intake generally exceeded ethanol intake in all lines, while P rats drank similar amounts of both fluids. These line differences indicate pleiotropic effects of genes that mediate ethanol intake and schedule-induced behaviors.

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Acknowledgement

Supported by National Institutes of Alcohol Abuse and Alcoholism grants AA07611, AA10722.

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Authors and Affiliations

  1. Department of Psychology, Program in Psychobiology of Addictions, Purdue School of Science, Indiana University/Purdue University at Indianapolis, Indianapolis, IN, 46202, USA

    N. W. Gilpin, N. E. Badia-Elder, R. L. Elder & R. B. Stewart

  2. The Committee on the Neurobiology of Addictive Disorders (CNAD), The Scripps Research Institute, SP30-2400, 10550 North Torrey Pines Rd., La Jolla, CA, 92037, USA

    N. W. Gilpin

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  1. N. W. Gilpin
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  2. N. E. Badia-Elder
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  3. R. L. Elder
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  4. R. B. Stewart
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Correspondence to N. W. Gilpin.

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Edited by Tamara Phillips.

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Gilpin, N.W., Badia-Elder, N.E., Elder, R.L. et al. Schedule-induced Polydipsia in Lines of Rats Selectively Bred for High and Low Ethanol Preference. Behav Genet 38, 515–524 (2008). https://doi.org/10.1007/s10519-008-9224-1

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  • DOI: https://doi.org/10.1007/s10519-008-9224-1

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