Abstract
Light with or without chemical agents has been used to induce therapeutic and antimicrobial effects. With photodynamic therapy, the antimicrobial effect is confined to areas covered by a photosensitive dye and irradiated with light. The aim of the present study was to evaluate the effect of photodynamic therapy for the treatment of halitosis in adolescents through the analysis of volatile sulfur compounds, especially sulfide. A controlled, clinical trial was conducted with 45 adolescents randomly allocated to three groups: group 1, photodynamic therapy administered to the dorsum of the tongue; group 2, treatment with a tongue scraper; and group 3, treatment with a tongue scraper combined with photodynamic therapy. The diagnosis of halitosis was performed using gas chromatography before and after treatment. Comparisons were made using the Kruskal-Wallis test followed by the Student-Newman-Keuls test, with the level of significance set at 5 % (p < 0.05). After treatment, a statistically significant reduction in halitosis was found in all groups (p < 0.001). The greatest reduction in total sulfides (median = 0) occurred with the combination of tongue scraper and photodynamic therapy. The present study describes a novel option for the treatment of halitosis in adolescents with an immediate effect that does not involve the mechanical aggression of the lingual papillae that occurs with conventional treatment.
Trial registration:
Photodynamic Therapy in Adolescents Halitosis (https://clinicaltrials.gov/ct2/show/NCT02007993?term=NCT02007993&rank=1)
Number: NCT02007993
Funding:
FAPESP
Number: 2013/13032-8
Similar content being viewed by others
References
Dai T, Fuchs BB, Coleman JJ, Prates RA, Astrakas C, St Denis TG et al (2012) Concepts and principles of photodynamic therapy as an alternative antifungal discovery platform. Front Microbiol 3:120
Liu P-F, Zhu W-H, Huang C-M (2009) Vaccines and photodynamic therapies for oral microbial-related diseases. Curr Drug Metab 10(1):90–4
Wilson M (2004) Lethal photosensitisation of oral bacteria and its potential application in the photodynamic therapy of oral infections. Photochem Photobiol Sci 3:412–8
Wainwright M (1998) Photodynamic antimicrobial chemotherapy (PACT). J Antimicrob Chemother 42:13–28
Shimura M, Watanabe S, Iwakura M, Oshikiri Y, Kusumoto M, Ikawa K et al (1997) Correlation between measurements using a new halitosis monitor and organoleptic assessment. J Periodontol 68(12):1182–5
Quirynen M, Avontroodt P, Soers C, Zhao H, Pauwels M, Van Steenberghe D (2004) Impact of tongue cleansers on microbial load and taste. J Clin Periodontol 31:506–10
Rosenberg M, McCulloch CA (1992) Measurement of oral malodor: current methods and future prospects. J Periodontol 63(9):776–82
Kizhner V, Xu D, Krespi YP (2011) A new tool measuring oral malodor quality of life. Eur Arch Otorhinolaryngol 268:1227–32
Amir E, Shimonov R, Rosenberg M (1999) Halitosis in children. J Pediatr 134(3):338–43
Bollen CML, Beikler T (2012) Halitosis: the multidisciplinary approach. Int J Oral Sci 4(2):55–63
Dal Rio AC, Passos CA, Nicola JH, Nicola EMD (2006) CO2 laser cryptolysis by coagulation for the treatment of halitosis. Photomed Laser Surg 24(5):630–6
Marocchio LS, da Conceição MD, Tárzia O (2009) Remoção da saburra lingual: comparação da eficiência de três técnicas. RGO 57:443–8
Quirynen M, Dadamio J, Van den Velde S, De Smit M, Dekeyser C, Van Tornout M et al (2009) Characteristics of 2000 patients who visited a halitosis clinic. J Clin Periodontol 36(11):970–5
Salako NO, Philip L (2011) Comparison of the use of the Halimeter and the Oral Chroma™ in the assessment of the ability of common cultivable oral anaerobic bacteria to produce malodorous volatile sulfur compounds from cysteine and methionine. Med Princ Pract 20(1):75–9
Tangerman A, Winkel EG (2008) The portable gas chromatograph OralChroma™: a method of choice to detect oral and extra-oral halitosis. J Breath Res 2(1)
Rosenberg M, Kulkarni G, Bosy A, McCulloch C (1991) Reproducibility and sensitivity of oral malodor measurements with a portable sulfide monitor. J Dent Res 70(11):1436–40
Tanabe S, Grenier D (2012) Characterization of volatile sulfur compound production by Solobacterium moorei. Arch Oral Biol 57(12):1639–43, Elsevier Ltd
Haraszthy VI, Gerber D, Clark B, Moses P, Parker C, Sreenivasan PK et al (2008) Characterization and prevalence of Solobacterium moorei associated with oral halitosis. J Breath Res 2(1):017002
Kara C, Demir T, Orbak R, Tezel A (2008) Effect of Nd: YAG laser irradiation on the treatment of oral malodour associated with chronic periodontitis. Int Dent J 58:151–8
Kara C, Tezel A, Orbak R (2006) Effect of oral hygiene instruction and scaling on oral malodour in a population of Turkish children with gingival inflammation. Int J Paediatr Dent 16(6):399–404
Collins LM, Dawes C (1987) The surface area of the adult human mouth and thickness of the salivary film covering the teeth and oral mucosa. J Dent Res 66(8):1300–2
Avcu N, Kanli A (2003) The prevalence of tongue lesions in 5150 Turkish dental outpatients. Oral Dis 188–95
Lopes R, Godoy C, Deana A, Santi M, Prates R, França C et al (2014) Photodynamic therapy as a novel treatment for halitosis in adolescents: study protocol for a randomized controlled trial. Trials 15(1):443
Pham T, Ueno M, Zaitsu T, Takehara S, Shinada K, Lam P et al (2011) Clinical trial of oral malodor treatment in patients with periodontal diseases. J Periodontal Res 46(6):722–9
Donaldson AC, Riggio MP, Rolph HJ, Bagg J, Hodge PJ (2007) Clinical examination of subjects with halitosis. Oral Dis 13(1):63–70
Lui J, Corbet E, Jin L (2011) Combined photodynamic and low-level laser therapies as an adjunct to nonsurgical treatment of chronic periodontitis. J Periodontal Res 46:89–96
Polansky R, Haas M, Heschl A, Wimmer G (2009) Clinical effectiveness of photodynamic therapy in the treatment of periodontitis. J Clin Periodontol 36:575–80
Lopes RG, Santi MESO, Franco BE, Deana AM, Prates RA, França CM et al (2014) Photodynamic therapy as novel treatment for halitosis in adolescents: a case series study. J Laser Med Sci 5(3):146–52
Seemann R, Conceição M, Filippi A, Greenman J, Lenton P, Nachnani S et al (2014) Halitosis management by the general dental practitioner—results of an international consensus workshop. J Breath Res 8(1):017101
Hamblin MR, Hasan T (2004) Photodynamic therapy: a new antimicrobial approach to infectious disease? Photochem Photobiol Sci 3(5):436–50
Dahl T, Midden W, Hartman P (1989) Comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. J Bacteriol 171:2188–94
Komerik N, MacRobert AJ (2006) Photodynamic therapy as an alternative antimicrobial modality for oral infections. J Environ Pathol Toxicol Oncol 25(1-2):487–504
Betsy J, Prasanth C, Baiju K, Prasanthila J, Subhash N (2014) Efficacy of antimicrobial photodynamic therapy in the management of chronic periodontitis: a randomized controlled clinical trial. J Clin Periodontol
Kleinberg I, Codipilly D (2002) Cysteine challenge testing: a powerful tool for examining oral malodour processes and treatments in vivo. Int Dent J 3:221–8
Demir T, Orbak R, Tezel A, Canakç V, Kaya H (2009) The changes in the T-lymphocyte subsets in a population of Turkish children with puberty gingivitis. Int J Paediatr Dent 19(3):206–12
Motta LJ, Bachiega JC, Guedes CC, Laranja LT, BussadoriI SK (2011) Association between halitosis and mouth breathing in children. Clinics 66(6):939–42
Acknowledgments
The cooperation of the Center for Excellence in the Treatment of Halitosis and the São Judas Tadeu Children’s Institute as well as the support from the FAPESP and Postgraduate Program in Biophotonics Applied to Health Sciences of University Nove de Julho, São Paulo, Brazil are greatly acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lopes, R.G., da Mota, A.C.C., Soares, C. et al. Immediate results of photodynamic therapy for the treatment of halitosis in adolescents: a randomized, controlled, clinical trial. Lasers Med Sci 31, 41–47 (2016). https://doi.org/10.1007/s10103-015-1822-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10103-015-1822-6