Abstract
The study conducted in a bacterial-based in vitro caries model aimed to determine whether typical inner secondary caries lesions can be detected at cavity walls of restorations with selected gap widths when the development of outer lesions is inhibited. Sixty bovine tooth specimens were randomly assigned to the following groups: test group 50 (TG50; gap, 50 μm), test group 100 (TG100; gap, 100 μm), test group 250 (TG250; gap, 250 μm) and a control group (CG; gap, 250 μm). The outer tooth surface of the test group specimens was covered with an acid-resistant varnish to inhibit the development of an outer caries lesion. After incubation in the caries model, the area of demineralization at the cavity wall was determined by confocal laser scanning microscopy. All test group specimens demonstrated only wall lesions. The CG specimens developed outer and wall lesions. The TG250 specimens showed significantly less wall lesion area compared to the CG (p < 0.05). In the test groups, a statistically significant increase (p < 0.05) in lesion area could be detected in enamel between TG50 and TG250 and in dentine between TG50 and TG100. In conclusion, the inner wall lesions of secondary caries can develop without the presence of outer lesions and therefore can be regarded as an entity on their own. The extent of independently developed wall lesions increased with gap width in the present setting.
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Acknowledgements
This study was supported by Marion von Zitzewitz and Steffie Balz who helped in the preparation of the specimens, Klaus Dannenberg from the “Medizinisch technische Labore” of the Charité by building the vice-like appliance, Dr. Carsten Grötzinger by helping us to take the CLSM images, Helmut Orawa from the “Institut für Biometrie und klinische Epidemiologie” of the Charité for his assistance in performing the statistical analysis. Special thanks to all of these people.
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Diercke, K., Lussi, A., Kersten, T. et al. Isolated development of inner (wall) caries like lesions in a bacterial-based in vitro model. Clin Oral Invest 13, 439–444 (2009). https://doi.org/10.1007/s00784-009-0250-z
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DOI: https://doi.org/10.1007/s00784-009-0250-z