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Clinical and microbiological effects of an essential-oil-containing mouth rinse applied in the “one-stage full-mouth disinfection” protocol—a randomized doubled-blinded preliminary study

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Abstract

The aim of this randomized double-blinded preliminary study was to evaluate the clinical and microbiological long-term effects of an essential-oil-containing mouth rinse as the active agent utilized in the “one-stage full-mouth disinfection protocol.” Probing pocket depth and plaque and gingival indices were evaluated by the same calibrated examiner in all teeth of 20 moderate chronic periodontitis subjects. Presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were determined by polymerase chain reaction in nonstimulated saliva, tongue dorsum, and pooled subgingival samples. The subjects were randomized into two groups: full-mouth disinfection plus essential oils (Listerine®) or full-mouth disinfection plus placebo. Clinical and microbial parameters were evaluated at baseline (T0), 45 (T1) and 180 (T2) days after therapy and analyzed using analysis of variance, Student t, and Wilcoxon tests (p < 0.05). No significant differences were observed between groups regarding clinical measurements at baseline. However, in the later examinations, T1 and T2, the test group always presented higher reductions of pocket depth, plaque index, and gingival index compared to the control group. The essential-oils group revealed significant reduction on occurrence of P. gingivalis in saliva comparing baseline and 45 days; this difference still remain at 180 days. The essential-oil-containing mouth rinse demonstrated beneficial effects on clinical parameters. Microbiological findings were less consistent. The results of this preliminary study suggest further investigations.

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Notes

  1. PCPUNC 15 Hu-friedy Mfg. Co. Inc., Chigago, IL, USA

  2. BioRad Laboratories, Hercules, CA, USA

  3. Invitrogen, USA

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Conflict of interests statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Periodontology and Preventive Dentistry, Dental Research Division, University of Taubaté, Rua Professor Nelson Freire Campelo, 343—Jardim Eulália, Taubaté, Sao Paolo, Brazil, 12010-700

    Sheila Cavalca Cortelli & José Roberto Cortelli

  2. Department of Periodontology, Dental Research Division, University of Taubaté, Taubaté, Sao Paolo, Brazil

    Fabiana Cavallini, Marcello Faria Regueira Alves & Arnaud Alves Bezerra Jr.

  3. Department of Oral Biology, Dental Research Division, University of Taubaté, Taubaté, Sao Paolo, Brazil

    Celso Silva Queiroz

Authors
  1. Sheila Cavalca Cortelli
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  2. Fabiana Cavallini
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  3. Marcello Faria Regueira Alves
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  4. Arnaud Alves Bezerra Jr.
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  5. Celso Silva Queiroz
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  6. José Roberto Cortelli
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Correspondence to Sheila Cavalca Cortelli.

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Cavalca Cortelli, S., Cavallini, F., Regueira Alves, M.F. et al. Clinical and microbiological effects of an essential-oil-containing mouth rinse applied in the “one-stage full-mouth disinfection” protocol—a randomized doubled-blinded preliminary study. Clin Oral Invest 13, 189–194 (2009). https://doi.org/10.1007/s00784-008-0219-3

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  • DOI: https://doi.org/10.1007/s00784-008-0219-3

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