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Smoking and AMH levels in women with normal reproductive history

  • Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To investigate the effect of age and smoking on the AMH levels in normal cycling healthy women with normal reproductive history.

Materials and methods

In 137 women, blood samples were taken on day 3 of a spontaneous cycle. Serum FSH, LH, E2, progesterone and AMH were measured in all blood samples. For the statistical analysis of the data, t test, Pearson’s correlation and linear regression analysis were performed.

Results

Of 137 women (43%), 59 were smokers. Age was positively correlated with serum FSH and LH levels (r = 0.584, P < 0.001 and r = 0.330, P < 0.001, respectively) and negatively correlated with serum AMH levels (r = −0.882, P < 0.001). There were no significant differences in FSH, LH, E2, progesterone and AMH levels between smokers and non-smokers. Multiple stepwise linear regression analysis showed that in both smokers and non-smokers, age was the most significant determinant of AMH levels (r = −0.889, P < 0.001 and r = −0.944, P < 0.001, respectively), while smoking was not related to AMH levels.

Conclusions

Aging significantly decreases AMH levels in women with normal cycles and normal reproductive history, while smoking does not seem to have significant effects on AMH levels.

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References

  1. Massague J, Wotton D (2000) Transcriptional control by the TGFbeta/Smad signaling system. EMBO J 19:1745–1754

    Article  CAS  PubMed  Google Scholar 

  2. Hughes IA (2001) Minireview: sex differentiation. Endocrinology 142:3281–3287

    Article  CAS  PubMed  Google Scholar 

  3. Broekmans F, Visser J, Laven J, Broer S, Themmen A, Fauser B (2008) Anti-Müllerian hormone and ovarian dysfunction. Trends Endocrinol Metab 19:340–347

    Article  CAS  PubMed  Google Scholar 

  4. Van Rooij IA, Broekmans FJ, te Velde ER, Fauser BC, Bancsi LF, Jong FH, Themmen AP (2002) Serum anti-Mullerian hormone levels: a novel measure of ovarian reserve. Hum Reprod 17:3065–3071

    Article  PubMed  Google Scholar 

  5. Broer S, Mol B, Hendriks D, Broekmans F (2009) The role of antimullerian hormone in prediction of outcome after IVF: comparison with the antral follicle count. Fertil Steril 91:705–714

    Article  CAS  PubMed  Google Scholar 

  6. The Practice Committee of the American Society for Reproductive Medicine (2004) Smoking and infertility. Fertil Steril 81:1181–1186

    Article  Google Scholar 

  7. Chang SH, Kim CS, Lee KS, Kim H, Yim SV, Lim YJ, Park SK (2007) Premenopausal factors influencing premature ovarian failure and early menopause. Maturitas 58:19–30

    Article  PubMed  Google Scholar 

  8. Soares SR, Velasco JA, Fernandez M, Bosch E, Remohí J, Pellicer A, Simón C (2008) Clinical factors affecting endometrial receptiveness in oocyte donation cycles. Fertil Steril 89:491–501

    Article  PubMed  Google Scholar 

  9. Freour T, Masson D, Mirallie S, Jean M, Baoh K, Dejoie T, Barriere P (2008) Active smoking compromises IVF outcome and affects ovarian reserve. RBM Online 16:96–102

    PubMed  Google Scholar 

  10. Waylen AL, Metwally M, Jones GL, Wilkinson AJ, Ledger WL (2009) Effects of cigarette smoking upon clinical outcomes of assisted reproduction: a meta-analysis. Hum Reprod Update 15:31–44

    Article  CAS  PubMed  Google Scholar 

  11. Nardo LG, Christodoulou D, Gould D, Roberts SA, Fitzgerald CT, Laing I (2007) Anti-Müllerian hormone levels and antral follicle count in women enrolled in in vitro fertilization cycles: relationship to lifestyle factors, chronological age and reproductive history. Gynecol Endocrinol 23:486–493

    Article  CAS  PubMed  Google Scholar 

  12. Lambert-Messerlian GM, Harlow BL (2006) The influence of depression, body mass index, and smoking on serum inhibin B levels in late reproductive-aged women. J Clin Endocrinol Metab 91:1496–1500

    Article  CAS  PubMed  Google Scholar 

  13. Sharara FI, Beatse SN, Leonardi MR, Navot D, Scott RT Jr (1994) Cigarette smoking accelerates the development of diminished ovarian reserve as evidenced by the clomiphene citrate challenge test. Fertil Steril 62:257–262

    CAS  PubMed  Google Scholar 

  14. Westhoff C, Murphy P, Heller D (2000) Predictors of ovarian follicle number. Fertil Steril 74:624–628

    Article  CAS  PubMed  Google Scholar 

  15. Zenzes MT (2000) Smoking and reproduction: gene damage to human gametes and embryos. Hum Reprod Update 6:122–131

    Article  CAS  PubMed  Google Scholar 

  16. Santoro N, Lasley B, McConnell D, Allsworth J, Crawford S, Gold EB, Finkelstein JS, Greendale GA, Kelsey J, Korenman S, Luborsky JL, Matthews K, Midgley R, Powell L, Sabatine J, Schocken M, Sowers MF, Weiss G (2004) Body size and ethnicity are associated with menstrual cycle alterations in women in the early menopausal transition: the Study of Women’s Health Across the Nation (SWAN) Daily Hormone Study. J Clin Endocrinol Metab 89:2622–2631

    Article  CAS  PubMed  Google Scholar 

  17. Zenzes MT, Puy LA, Bielecki R (1997) Immunodetection of cotinine protein in granulosa-lutein cells of women exposed to cigarette smoke. Fertil Steril 68:76–82

    Article  CAS  PubMed  Google Scholar 

  18. Gocze PM, Szabo I, Freeman DA (1999) Influence of nicotine, cotinine, anabasine and cigarette smoke extract on human granulosa cell progesterone and estradiol synthesis. Gynecol Endocrinol 13:266–272

    Article  CAS  PubMed  Google Scholar 

  19. Barbieri RL, McShane PM, Ryan KJ (1996) Constituents of cigarette smoke inhibit granulosa cell aromatase. Fertil Steril 46:232–236

    Google Scholar 

  20. Bódis J, Hanf V, Török A, Tinneberg HR, Borsay P, Szabó I (1997) Influence of nicotine on progesterone and estradiol production of cultured human granulosa cells. Early Pregnancy 3:34–37

    PubMed  Google Scholar 

  21. Weiss T, Eckert A (1989) Cotinine levels in follicular fluid and serum of IVF patients: effect on granulosa-luteal cell function in vitro. Hum Reprod 4:482–485

    CAS  PubMed  Google Scholar 

  22. Sanders SR, Cuneo SP, Turzillo AM (2002) Effects of nicotine and cotinine on bovine theca interna and granulosa cells. Reprod Toxicol 16:795–800

    Article  CAS  PubMed  Google Scholar 

  23. Paszkowski T, Clarke RN, Hornstein MD (2002) Smoking induces oxidative stress inside the Graafian follicle. Hum Reprod 17:921–925

    Article  CAS  PubMed  Google Scholar 

  24. Bordel R, Laschke MW, Menger MD, Vollmar B (2006) Nicotine does not affect vascularization but inhibits growth of freely transplanted ovarian follicles by inducing granulosa cell apoptosis. Hum Reprod 21:610–617

    Article  CAS  PubMed  Google Scholar 

  25. Weinberg CR, Wilcox AJ, Baird DD (1989) Reduced fecundability in women with prenatal exposure to cigarette smoking. Am J Epidemiol 129:1072–1078

    CAS  PubMed  Google Scholar 

  26. Jurisicova A, Taniuchi A, Li H, Shang Y, Antenos M, Detmar J, Xu J, Matikainen T, Benito Hernández A, Nunez G, Casper RF (2007) Maternal exposure to polycyclic aromatic hydrocarbons diminishes murine ovarian reserve via induction of Harakiri. J Clin Invest 117:3971–3978

    CAS  PubMed  Google Scholar 

  27. de Vet A, Laven JS, de Jong FH, Themmen AP, Fauser BC (2002) Antimüllerian hormone serum levels: a putative marker for ovarian aging. Fertil Steril 77:357–362

    Article  PubMed  Google Scholar 

  28. La Marca A, Sighinolfi G, Radi D, Argento C, Baraldi E, Artenisio AC, Stabile G, Volpe A (2009) Anti-Mullerian hormone (AMH) as a predictive marker in assisted reproductive technology (ART). Hum Reprod Update 16:113–130

    Google Scholar 

  29. Baarends WM, Uilenbroek JT, Kramer P, Hoogerbrugge JW, van Leeuwen EC, Themmen AP, Grootegoed JA (1995) Anti-Müllerian hormone and anti-Müllerian hormone type II receptor messenger ribonucleic acid expression in rat ovaries during postnatal development, the estrous cycle and gonadotropin-induced follicle growth. Endocrinology 136:4951–4962

    Article  CAS  PubMed  Google Scholar 

  30. Lenton EA, Sexton L, Lee S, Cooke ID (1998) Progressive changes in LH and FSH and LH: FSH ratio in women throughout reproductive life. Maturitas 10:35–43

    Article  Google Scholar 

  31. Randolph JF Jr, Sowers M, Bondarenko IV, Harlow SD, Luborsky JL, Little RJ (2004) Change in estradiol and follicle-stimulating hormone across the early menopausal transition: effects of ethnicity and age. J Clin Endocrinol Metab 89:1555–1561

    Article  CAS  PubMed  Google Scholar 

  32. Santoro N, Brown JR, Adel T, Skurnick JH (1996) Characterization of reproductive hormonal dynamics in the perimenopause. J Clin Endocrinol Metab 81:1495–1501

    Article  CAS  PubMed  Google Scholar 

  33. Messinis IE (2006) Ovarian feedback, mechanism of action and possible clinical implications. Hum Reprod Update 12:557–571

    Article  CAS  PubMed  Google Scholar 

  34. Pasquali R (2006) Obesity and androgens: facts and perspectives. Fertil Steril 85:1319–1340

    Article  CAS  PubMed  Google Scholar 

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The authors declare that they have no conflict of interest.

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Correspondence to Konstantinos Dafopoulos.

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Dafopoulos, A., Dafopoulos, K., Georgoulias, P. et al. Smoking and AMH levels in women with normal reproductive history. Arch Gynecol Obstet 282, 215–219 (2010). https://doi.org/10.1007/s00404-010-1425-1

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  • DOI: https://doi.org/10.1007/s00404-010-1425-1

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