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01-05-2008 | original article

Progenitor cells isolated from the human heart: a potential cell source for regenerative therapy

Auteurs: P. van Vliet, M. Roccio, A. M. Smits, A. A. M. van Oorschot, C. H. G. Metz, T. A. B. van Veen, J. P. G. Sluijter, P. A. Doevendans, M-J. Goumans

Gepubliceerd in: Netherlands Heart Journal | Uitgave 5/2008

Abstract

Background. In recent years, resident cardiac progenitor cells have been identified in, and isolated from the rodent heart. These cells show the potential to form cardiomyocytes, smooth muscle cells, and endothelial cells in vitro and in vivo and could potentially be used as a source for cardiac repair. However, previously described cardiac progenitor cell populations show immature development and need co-culture with neonatal rat cardiomyocytes in order to differentiate in vitro. Here we describe the localisation, isolation, characterisation, and differentiation of cardiomyocyte progenitor cells (CMPCs) isolated from the human heart.

Methods. hCMPCs were identified in human hearts based on Sca-1 expression. These cells were isolated, and FACS, RT-PCR and immunocytochemistry were used to determine their baseline characteristics. Cardiomyogenic differentiation was induced by stimulation with 5-azacytidine.

Results. hCMPCs were localised within the atria, atrioventricular region, and epicardial layer of the foetal and adult human heart. In vitro, hCMPCs could be induced to differentiate into cardiomyocytes and formed spontaneously beating aggregates, without the need for co-culture with neonatal cardiomyocytes.

Conclusion. The human heart harbours a pool of resident cardiomyocyte progenitor cells, which can be expanded and differentiated in vitro. These cells may provide a suitable source for cardiac regeneration cell therapy. (Neth Heart J 2008;16: 163-9.)

vorige artikel Characteristics of the flow velocity-pressure gradient relation in the assessment of stenoses: an in vitro study

volgende artikel Recovery of atrial transport function after a maze procedure for atrial fibrillation in conversion of a failing Fontan circulation

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Titel: Progenitor cells isolated from the human heart: a potential cell source for regenerative therapy
Auteurs: P. van Vliet
M. Roccio
A. M. Smits
A. A. M. van Oorschot
C. H. G. Metz
T. A. B. van Veen
J. P. G. Sluijter
P. A. Doevendans
M-J. Goumans
Publicatiedatum: 01-05-2008
Uitgeverij: Bohn Stafleu van Loghum
Gepubliceerd in: Netherlands Heart Journal / Uitgave 5/2008
Print ISSN: 1568-5888
Elektronisch ISSN: 1876-6250
DOI: https://doi.org/10.1007/BF03086138

Bohn Stafleu van Loghum

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