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Immunological and virological investigations in Down's syndrome

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Abstract

Lymphocyte responsiveness to phytohaemagglutinin and viral antigens was studied in children with Down's syndrome and in controls. Mitogen-responsiveness in the patients was significantly reduced as compared to the control values. Using the lymphocyte transformation test, trisomic patients showed more than a twofold increase in sensitivity to herpes simplex virus as compared to controls. The same test did not show any essential difference between the two groups when adeno- and influenza viruses were used. Immunofluorescence technique, with specifically conjugated antiviral sera, permitted the detection of specific fluorescence in 30% of the patients with Down's syndrome indicating the presence of oncogenic adenovirus type 12 antigen in the circulating lymphocytes. No antibodies—or only very low titres—against adeno- and herpes simplex viruses were demonstrated in the sera of trisomic patients. Mononuclear leukocytes from these patients often showed structural alterations. The incidence of infectious herpes simplex virus and Candida albicans in the saliva of patients was higher than in the control group.

It seems that Down's syndrome involves partial disturbance of both the cellular and humoral immune functions—at least with respect to certain viral antigens.

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References

  1. Agarwal SS, Baruch S, Blumberg J, Gerstley BJ, London WT, Sutnick AJ, Loeb LA (1970) DNA polymerase activity as an index of lymphocyte stimulation: Studies in Down's syndrome. J Clin Invest 49:161–169

    Google Scholar 

  2. Allison AC (1972) Immunity and immunpathology in virus infections. Ann Inst Pasteur 123:585–608

    Google Scholar 

  3. Burgio GR, Ugazio AG, Nespoli L, Marcioni AF, Bottelli AM, Pasquali F (1975) Derangements of immunoglobulin levels, phytohemagglutinin responsiveness and T and B cell markers in Down's syndrome at different ages. Eur J Immunol 5:600–603

    Google Scholar 

  4. Campion EC, Wangel AG (1975) Australia antigens, antibodies and cell mediated immune responses in patients with Down's syndrome. Med J Austr 15:468–470

    Google Scholar 

  5. Leading article (1973) Cell-mediated immunity and resistance to infection. WHO Chronicle 27:531–533

    Google Scholar 

  6. Coons AB, Kaplan KH (1950) Localisation of antigen in tissue cells. Improvements in method for detection of antigen by means of fluorescent antibody. J Exp Med 91:1–13

    Google Scholar 

  7. Dám P, Kulcsár G, Sallay K, Nász I (1971) Human lymphocyte transformation with virus antigens. Blut 22:211–215

    Google Scholar 

  8. Garfinkle B, McAuslan BR (1974) Transformation of cultured mammalian cells by viable herpes simplex virus subtypes 1 and 2. Proc Nat Acad Sci USA 71:220–224

    Google Scholar 

  9. Gordon MC, Sinha SK, Carlson SD (1971) Antibody responses to influenza vaccine in patients with Down's syndrome. Amer J Mental Defic 75:391–399

    Google Scholar 

  10. Gresser J, Lang JD (1966) Relationship between viruses and leukocytes. Progr Med Virol 8:62–130

    Google Scholar 

  11. Halpern B, Ky NT, Lagrue G, Amache N (1967) Le test aux lymphocytes en allergologie. Méd et Hyg 25:55–57

    Google Scholar 

  12. Hawkes RA, Boughton CR, Schroeter DR, Decker RH, Overby RL (1980) Hepatitis B infection in institutionalized Down's syndrome inmates: a longitudinal study with five hepatitis B virus markers. Clin Exp Immunol 40:478–486

    Google Scholar 

  13. Klein G (1972) Herpes virus and oncogenesis. Proc Nat Acad Sci USA 69:1056–1064

    Google Scholar 

  14. Kraft SC, Kirsner JB (1971) Immunological apparatus of the gut and inflammatory bowel disease. Gastroenterology 60:922–951

    Google Scholar 

  15. Kulcsár G (1975) Persistáló adeno- és herpes simplex virusok a human pathológiaban. Thesis, Budapest

  16. Levin S, Nir E, Mogilner BM (1975) T system immune-deficiency in Down's syndrome. Pediatrics 56:123–127

    Google Scholar 

  17. Lopez V, Millar A, Thuline HC (1973) Antibody deficiency in trisomy 21. (Abst) Clin Res 21:212

    Google Scholar 

  18. Lopez V, Ochs HD, Thuline HC, Davis SD, Wedgwood RJ (1975) Defective antibody response to bacteriophage ØX 174 in Down syndrome. J Pediatr 86:207–211

    Google Scholar 

  19. Madrafo JL, Tampavillas M, Bill M (1974) Severe combined immunodeficiency associated with Down's syndrome. Ann Exp Pediatr 7:429–437

    Google Scholar 

  20. Mellmann WJ, Younkin LH, Baker D (1970) Abnormal lymphocyte function in trisomy 21. Ann NY Acad Sci 171:537–542

    Google Scholar 

  21. Miller RW (1970) Neoplasia and Down's syndrome. Ann NY Acad Sci 171:637–644

    Google Scholar 

  22. Nasemann T (1965) Die Infektionen durch das Herpes simplex-Virus; Monographie. Fischer Verlag, Jena

    Google Scholar 

  23. Nász I, Béládi I, Lengyel A (1967) Az adenovirusok és kórokozó szerepük. Akadémiai Kiadó, Budapest

    Google Scholar 

  24. Nász I, Kulcsár G, Dán P, Sallay K, Vértes L, Geck P, Keskeny S, Horváth J (1973) Aetiological significance of viruses and role of lymphocytes in certain gastrointestinal diseases. Acta Microbiol Acad Sci Hung 20:191–203

    Google Scholar 

  25. Nász I, Berencsi Gy (1975) Az adenovirus DNS molekulárbiológiai tulajdonságai és daganatkeltö képessége. Orv Hetil 116:1503–1508

    Google Scholar 

  26. Rapp F, Duff R (1973) Transformation of hamster embryo fibroblasts by herpes simplex viruses types 1 and 2. Cancer Res 33:1526–1534

    Google Scholar 

  27. Sallay K, Dán P, Geck P, Kulcsár G, Nász I (1971) Immunofluorescent studies in circulating lymphocytes in oral mucosal diseases. Arch Derm Forsch 241:15–21

    Google Scholar 

  28. Sutnick A, London WT, Blumberg BS, Gerstley BJS (1971) Susceptibility to leukaemia: Immunologic factors in Down's syndrome. J Nat Cancer Inst 47:923–933

    Google Scholar 

  29. Szigeti R, Révész T, Schuler D (1974) Celluláris immunitási vizsgálatok Down-kóros gyermekeken. Orv Hetil 115:2173–2175

    Google Scholar 

  30. Takátsy Gy (1955) The use of spiral loops in serological and virological micro-methods. Acta Microbiol Acad Sci Hung 3:191–196

    Google Scholar 

  31. Todaro GJ, Martin GM (1967) Increased susceptibility of Down's syndrome fibroblasts to transformation by SV 40 (31974). Proc Soc Exp Biol Med 124:1232–1236

    Google Scholar 

  32. Wheelock EF, Toy ST (1973) Participation of lymphocytes in viral infections. Adv Immunol 16:123–184

    Google Scholar 

  33. Whittingham S, Sharma DLB, Pitt DB, Mackay IR (1977) Stress deficiency of the T-lymphocyte system exemplified by Down syndrome. Lancet I:163–166

    Google Scholar 

  34. Wood TA (1967) The atypical lymphocytes. Amer J Med 42:923–936

    Google Scholar 

  35. Woodruff JF, Woodruff JJ (1975) T-lymphocyte interaction with viruses and virus-infected tissues. Progr Med Virol 19:120–160

    Google Scholar 

  36. Woods LK, Moore GE, Bainbridge CJ, Huang CC, Huzella C, Quinn LA (1973) Lymphoid cell lines. Established from peripheral blood of persons with Down's syndrome. NY State J Med 73:869–873

    Google Scholar 

  37. Zuckermann AJ (1970) Viral hepatitis and tests for the Australia (Hepatitis-Associated) antigen and antibody. WHO Memoranda, 957–992

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Authors and Affiliations

  1. Second Department of Paediatrics, IX., Tüzoltó u. 7/9., 1094, Budapest, Hungary

    G. Fekete, G. Kulcsár, P. Dán, I. Nász, D. Schuler & Matild Dobos

  2. Department of Microbiology, Semmelweis University Medical School, Budapest, Hungary

    G. Fekete, G. Kulcsár, P. Dán, I. Nász, D. Schuler & Matild Dobos

Authors
  1. G. Fekete
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  2. G. Kulcsár
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  3. P. Dán
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  4. I. Nász
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  5. D. Schuler
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  6. Matild Dobos
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Fekete, G., Kulcsár, G., Dán, P. et al. Immunological and virological investigations in Down's syndrome. Eur J Pediatr 138, 59–62 (1982). https://doi.org/10.1007/BF00442331

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  • DOI: https://doi.org/10.1007/BF00442331

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