This study aims to develop a self-report measure based on a multidimensional conceptualization of the regulation of negative emotions in the context of parenting. We examined the factor structure, factorial invariance, internal consistency and construct validity of the newly developed measure, the Parent Emotion Regulation Scale (PERS). The sample was composed of 254 mothers of children aged 3 to 15 years old (M = 8.54, SD = 3.53) who participated in an online study. Exploratory factor analysis of the PERS yielded a four-factor structure with the following factors: parents’ orientation to the child’s emotions, parents’ avoidance of the child’s emotions, parents’ lack of emotional control and parents’ acceptance of the child’s and their own emotions. Confirmatory factorial analysis supported the four-factor model, showing an adequate fit for this structure. Additionally, the model displayed configural, metric, scalar and residual invariance across the children’s sex groups. Cronbach’s alpha values and mean inter-item correlations indicated an adequate internal consistency. Correlations between the PERS scale and the Coping with Children’s Negative Emotions Scale (CCNES) indicated that the different dimensions of parents’ emotion regulation were significantly associated with both positive and negative reactions to the children’s negative emotions. Furthermore, the emotion regulation dimensions showed incremental validity in the prediction of the child’s adjustment. This study provides preliminary support for the adequacy of this newly developed measure, although further studies are needed to establish its psychometric qualities in more diverse populations.