This study examined the factor structure and test-retest invariance, and temporal stability and uniqueness of the latent factors (depression, anxiety, and stress) of the Depression Anxiety Stress Scales-21 (DASS-21; Lovibond & Lovibond, 1995) in group a of 269 older adults (age ranging from 60 to 85 years) from the general community. Participants completed the DASS-21 twice, 3 months apart. Confirmatory factor analysis (CFA) of their ratings at Time 1 indicated support for the original 3-factor oblique model (factors for depression, anxiety, and stress). Additional analyses showed support for test-retest invariance for both the measurement (configural, metric and thresholds) and structural (variances and covariances) components of this model. Results also indicated temporal stability and uniqueness of the latent factors. The practical, theoretical, research and clinical implications of the findings are discussed.