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Methods of Assessing Human Tendon Metabolism and Tissue Properties in Response to Changes in Mechanical Loading

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Metabolic Influences on Risk for Tendon Disorders

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 920))

Abstract

In recent years a number of methodological developments have improved the opportunities to study human tendon. Microdialysis enables sampling of interstitial fluid in the peritendon tissue, while sampling of human tendon biopsies allows direct analysis of tendon tissue for gene- and protein expression as well as protein synthesis rate. Further the 14C bomb-pulse method has provided data on long-term tissue turnover in human tendon. Non-invasive techniques allow measurement of tendon metabolism (positron emission tomography (PET)), tendon morphology (magnetic resonance imaging (MRI)), and tendon mechanical properties (ultrasonography combined with force measurement during movement). Finally, 3D cell cultures of human tendon cells provide the opportunity to investigate cell-matrix interactions in response to various interventions.

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Abbreviations

Asp:

Aspartate

CSA:

Cross-sectional area

FGD:

Fluorodeoxyglucose

MMP:

Matrix metallo proteinase

MRI:

Magnetic resonance imaging

PET:

Positron emission tomography

PINP:

Pro-collagen I N-terminal peptide

PICP:

Pro-collagen I C-terminal peptide

ICTP:

Human C-telopeptide of type I collagen

UTC:

Ultrasound Tissue Characterization

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Author information

Authors and Affiliations

  1. Institute of Sports Medicine, Department of Orthopedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark

    Katja M. Heinemeier & Michael Kjaer

  2. Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Michael Kjaer

  3. Department of Physical Therapy, Musculoskeletal Rehabilitation Research Unit, Bispebjerg Hospital, Copenhagen, Denmark

    S. Peter Magnusson

  4. Department of Biomedical Sciences, Centre for Healthy Ageing, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Katja M. Heinemeier

  5. Institute of Sports Medicine, Department of Orthopedic Surgery M, Bispebjerg Hospital, Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    S. Peter Magnusson

Authors
  1. Katja M. Heinemeier
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  2. Michael Kjaer
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  3. S. Peter Magnusson
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Correspondence to Katja M. Heinemeier .

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Editors and Affiliations

  1. Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden

    Paul W. Ackermann

  2. McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, Alberta, Canada

    David A. Hart

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Heinemeier, K.M., Kjaer, M., Magnusson, S.P. (2016). Methods of Assessing Human Tendon Metabolism and Tissue Properties in Response to Changes in Mechanical Loading. In: Ackermann, P., Hart, D. (eds) Metabolic Influences on Risk for Tendon Disorders. Advances in Experimental Medicine and Biology, vol 920. Springer, Cham. https://doi.org/10.1007/978-3-319-33943-6_8

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