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Stem Cells and Organoids to Study Epithelial Cell Biology in IBD

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Crohn's Disease and Ulcerative Colitis

Abstract

Human intestinal organoids are three-dimensional tissue structures with a high degree of cellular and architectural complexity similar to the intestine. The identification of Lgr5-expressing adult intestinal stem cells was a seminal advance in the establishment of adult intestinal organoids (also called enteroids), which can be derived from patient biopsies and used to model human diseases. A second type of intestinal organoid that is generated via the directed differentiation of human pluripotent stem cells (hPSCs), into induced human intestinal organoids (iHIOs), has also proven useful in modeling developmental and postnatal diseases of the GI tract. Here we describe the various intestinal organoids systems, and how they have been used to study epithelial cell biology in the context of inflammatory bowel disease (IBD). In addition, we examine the advantages and disadvantages of the different organoid systems. Finally, we discuss emerging technologies and how they may be used in the future for drug development and as therapeutics.

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Authors and Affiliations

  1. Division of Developmental Biology, Cincinnati Children’s Hospital Research Foundation, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA

    Jorge O. Múnera & James M. Wells Ph.D.

Authors
  1. Jorge O. Múnera
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  2. James M. Wells Ph.D.
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Correspondence to James M. Wells Ph.D. .

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Editors and Affiliations

  1. Inflammatory Bowel Disease Center, Department of Gastroenterology and Hepatology, Charité Medical School—Humboldt-University of Berlin, Berlin, Germany

    Daniel C. Baumgart

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Múnera, J.O., Wells, J.M. (2017). Stem Cells and Organoids to Study Epithelial Cell Biology in IBD. In: Baumgart, D. (eds) Crohn's Disease and Ulcerative Colitis. Springer, Cham. https://doi.org/10.1007/978-3-319-33703-6_16

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  • DOI: https://doi.org/10.1007/978-3-319-33703-6_16

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-33701-2

  • Online ISBN: 978-3-319-33703-6

  • eBook Packages: MedicineMedicine (R0)

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