Skip to main content
SpringerLink
Log in

Merging Structure and Function: Combination of In Vivo Extracellular and Intracellular Electrophysiological Recordings with Neuroanatomical Techniques

  • Chapter
Neuroanatomical Tract-Tracing 3

Abstract

In order to understand the involvement of activity of single neurons in the context of the activity generated in small or larger neuronal networks, electrophysiological methods and morphological techniques need to be combined. In this chapter I describe a combination of methods designed to enable researchers to record the electrophysiological activity of single neurons in the intact brain, to analyze the interactions of these identified neurons with the surrounding neuronal network, and to investigate afterward the neuroanatomical characteristics of the recorded neurons.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bartho, P., Hirase, H., Monconduit, L., Zugaro, M., Harris, K. D., and Buzsaki, G., 2004, Characterization of neocortical principal cells and interneurons by network interactions and extracellular features, J. Neurophysiol. 92:600–608.

    Article  PubMed  Google Scholar 

  • Bragin, A., Jando, G., Nadasdy, Z., Hetke, J., Wise, K., and Buzsaki, G., 1995, Gamma (40–100 Hz) oscillation in the hippocampus of the behaving rat, J. Neurosci. 15:47–60.

    PubMed  CAS  Google Scholar 

  • Buzsaki, G., 2004, Large-scale recording of neuronal ensembles, Nat. Neurosci. 7:446–451.

    Article  PubMed  CAS  Google Scholar 

  • Buzsaki, G., Hazan, L., Zugaro, M. B., and Csicsvari, J., 2004, Neuroscope: a viewer for neurophysiological data, Soc. Neurosci. (abstract):768.2.

    Google Scholar 

  • Buzsaki, G., and Kandel, A., 1998, Somadendritic backpropagation of action potentials in cortical pyramidal cells of the awake rat, J. Neurophysiol. 79:1587–1591.

    PubMed  CAS  Google Scholar 

  • Buzsáki, G., Traub, R., and Pedley, T., 2003, The cellular synaptic generation of EEG. In: Ebersole, J.S. and Pedley, T.A. (eds.), Current Practice of Clinical Encephelography, Philadelphia: Lippincott-Williams and Wilkins, (3rd ed.), pp. 1–11.

    Google Scholar 

  • Chang, H. T., Wilson, C. J., and Kitai, S. T., 1981, Single neostriatal efferent axons in the globus pallidus: a light and electron microscopic study, Science 213:915–918.

    Article  PubMed  CAS  Google Scholar 

  • Csicsvari, J., Henze, D. A., Jamieson, B., Harris, K. D., Sirota, A., Bartho, P., Wise, K. D., and Buzsaki, G., 2003, Massively parallel recording of unit and local field potentials with siliconbased electrodes, J. Neurophysiol. 90:1314–1323.

    Article  PubMed  Google Scholar 

  • Cuello, A. C., 1993, Immunohistochemistry II, IBRO handbook series: Methods in the neurosciences. John Wiley & Sons, England.

    Google Scholar 

  • Ferster, D., and Jagadeesh, B., 1992, EPSP-IPSP interactions in cat visual cortex studied with in vivo whole-cell patch recording, J. Neurosci. 12:1262–1274.

    PubMed  CAS  Google Scholar 

  • Freund, T. F., and Buzsaki, G., 1996, Interneurons of the hippocampus, Hippocampus 6:347–470.

    Article  PubMed  CAS  Google Scholar 

  • Gray, C. M., Maldonado, P. E., Wilson, M., and McNaughton, B. L., 1995, Tetrodes markedly improve the reliability and yield of multiple single-unit recordings in cat striate cortex, J. Neurosci. Methods 63:43–54.

    Article  PubMed  CAS  Google Scholar 

  • Grossman, R. G., and Hampton, T., 1968, Depolarization of cortical glial cells during electrocortical activity, Brain Res. 11:316–324.

    Article  PubMed  CAS  Google Scholar 

  • Halasy, K., Buhl, E. H., Lorinczi, Z., Tamas, G., and Somogyi, P., 1996, Synaptic target selectivity and input of GABAergic basket and bistratified interneurons in the CA1 area of the rat hippocampus, Hippocampus 6:306–329.

    Article  PubMed  CAS  Google Scholar 

  • Harris, K. D., Henze, D. A., Csicsvari, J., Hirase, H., and Buzsaki, G., 2000, Accuracy of tetrode separation as determined by simultaneous intracellular and extracellular measurements, J. Neurophysiol. 84:401–414.

    PubMed  CAS  Google Scholar 

  • Hassin, G., 1979, Pikeperch horizontal cells identified by intracellular staining, J. Comp. Neurol. 186:529–540.

    Article  PubMed  CAS  Google Scholar 

  • Hazan, L., Zugaro, M. B., Csicsvari, J., Creese, I., and Buzsáki, G., 2004, Klusters: a graphical application for spike sorting of extracellular units, Soc. Neurosci. (abstract):768.3.

    Google Scholar 

  • Heimer, L., and Robards, M. J., 1981, Neuroanatomical Tract Tracing Methods, New York: Plenum Press.

    Google Scholar 

  • Henze, D. A., Borhegyi, Z., Csicsvari, J., Mamiya, A., Harris, K. D., and Buzsaki, G., 2000, Intracellular features predicted by extracellular recordings in the hippocampus in vivo, J. Neurophysiol. 84:390–400.

    PubMed  CAS  Google Scholar 

  • Horikawa, K., and Armstrong, W. E., 1988, A versatile means of intracellular labeling: injection of biocytin and its detection with avidin conjugates, J. Neurosci. Methods 25:1–11.

    Article  PubMed  CAS  Google Scholar 

  • Hubel, D. H., 1957, Tungsten microelectrodes for recording single units, Science 125: 549–550.

    Article  Google Scholar 

  • Jankowska, E., Rastad, J., and Westman, J., 1976, Intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells, Brain Res. 105:557–562.

    Article  PubMed  CAS  Google Scholar 

  • Kamondi, A., Acsady, L., and Buzsaki, G., 1998, Dendritic spikes are enhanced by cooperative network activity in the intact hippocampus, J. Neurosci. 18:3919–3928.

    PubMed  CAS  Google Scholar 

  • Kaneko, A., 1970, Physiological and morphological identification of horizontal, bipolar and amacrine cells in goldfish retina, J. Physiol. 207:623–633.

    PubMed  CAS  Google Scholar 

  • Kawaguchi, Y., 1993, Physiological, morphological, and histochemical characterization of three classes of interneurons in rat neostriatum, J. Neurosci. 13:4908–4923.

    PubMed  CAS  Google Scholar 

  • Kawaguchi, Y., Wilson, C. J., and Emson, P. C., 1989, Intracellular recording of identified neostriatal patch and matrix spiny cells in a slice preparation preserving cortical inputs, J. Neurophysiol. 62:1052–1068.

    PubMed  CAS  Google Scholar 

  • Kawaguchi, Y., Wilson, C. J., and Emson, P. C., 1990, Projection subtypes of rat neostriatal matrix cells revealed by intracellular injection of biocytin, J. Neurosci. 10:3421–3438.

    PubMed  CAS  Google Scholar 

  • Kelly, J. P., and Van Essen, D. C., 1974, Cell structure and function in the visual cortex of the cat, J. Physiol. 238:515–547.

    PubMed  CAS  Google Scholar 

  • Kita, H., and Armstrong, W., 1991, A biotin-containing compound N-(2-aminoethyl)biotinamide for intracellular labeling and neuronal tracing studies: comparison with biocytin, J. Neurosci. Methods 37:141–150.

    Article  PubMed  CAS  Google Scholar 

  • Kita, H., and Kitai, S. T., 1986, Electrophysiology of rat thalamo-cortical relay neurons: an in vivo intracellular recording and labeling study, Brain Res. 371:80–89.

    Article  PubMed  CAS  Google Scholar 

  • Kitai, S. T., Kocsis, J. D., Preston, R. J., and Sugimori, M., 1976, Monosynaptic inputs to caudate neurons identified by intracellular injection of horseradish peroxidase, Brain Res. 109:601–606.

    Article  PubMed  CAS  Google Scholar 

  • Lee, M. G., Hassani, O. K., Alonso, A., and Jones, B. E., 2005, Cholinergic basal forebrain neurons burst with theta during waking and paradoxical sleep, J. Neurosci. 25:4365–4369.

    Article  PubMed  CAS  Google Scholar 

  • Lee, M. G., Manns, I. D., Alonso, A., and Jones, B. E., 2004, Sleep-wake related discharge properties of basal forebrain neurons recorded with micropipettes in head-fixed rats, J. Neurophysiol. 92:1182–1198.

    Article  PubMed  Google Scholar 

  • Li, X.-G., Somogyi, P., Tepper, J. M., and Buzsaki, G., 1992, Axonal and dendritic arborization of an intracellularly labeled chandelier cell in the CA1 region of rat hippocampus, Exp. Brain Res. 90:519–525.

    Article  PubMed  CAS  Google Scholar 

  • Li, X. G., Somogyi, P., Ylinen, A., and Buzsaki, G., 1993, The hippocampal CA3 network: an in vivo intracellular labeling study, J. Comp. Neurol. 339:181–208.

    Article  Google Scholar 

  • Margrie, T.W., Brecht, M., and Sakmann, B., 2002, In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain, Pflugers Arch. 444:491–498.

    Article  PubMed  CAS  Google Scholar 

  • McNaughton, B. L., O’Keefe, J., and Barnes, C. A., 1983, The stereotrode: a new technique for simultaneous isolation of several single units in the central nervous system from multiple unit records, J. Neurosci. Methods 8:391–397.

    Article  PubMed  CAS  Google Scholar 

  • Nadasdy, Z., Csicsvari, J., Penttonen, M., and Buzsaki, G., 1998, Extracellular recording and analysis of electrical activity: from single cells to ensembles. In: Eichenbaum, H., and Davis, J. L. (eds.), Neuronal Ensembles: Strategies for Recording and Decoding, New York: Wiley-Liss, pp. 17–55.

    Google Scholar 

  • Sandell, J. H., and Masland, R. H., 1988, Photoconversion of some fluorescent markers to a diaminobenzidine product, J. Histochem. Cytochem. 36:555–559.

    PubMed  CAS  Google Scholar 

  • Sik, A., Penttonen, M., and Buzsaki, G., 1997, Interneurons in the hippocampal dentate gyrus: an in vivo intracellular study, Eur. J. Neurosci. 9:573–588.

    Article  PubMed  CAS  Google Scholar 

  • Sik, A., Penttonen, M., Ylinen, A., and Buzsaki, G., 1995, Hippocampal CA1 interneurons: an in vivo intracellular labeling study, J. Neurosci. 15:6651–6665.

    PubMed  CAS  Google Scholar 

  • Sik, A., Tamamaki, N., and Freund, T. F., 1993, Complete axon arborization of a single CA3 pyramidal cell in the rat hippocampus, and its relationship with postsynaptic parvalbumincontaining interneurons, Eur. J. Neurosci. 5:1719–1728.

    Article  PubMed  CAS  Google Scholar 

  • Sik, A., Ylinen, A., Penttonen, M., and Buzsaki, G., 1994, Inhibitory CA1-CA3-hilar region feedback in the hippocampus, Science 265:1722–1724.

    Article  PubMed  CAS  Google Scholar 

  • Simons, D. J., 1978, Response properties of vibrissa units in rat SI somatosensory neocortex, J. Neurophysiol. 41:798–820.

    PubMed  CAS  Google Scholar 

  • Snow, P. J., Rose, P. K., and Brown, A. G., 1976, Tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase, Science 191:312–313.

    Article  PubMed  CAS  Google Scholar 

  • Stewart, W. W., 1978, Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer, Cell 14:741–759.

    Article  PubMed  CAS  Google Scholar 

  • Stewart, W. W., 1981, Lucifer dyes-highly fluorescent dyes for biological tracing, Nature 292:17–21.

    Article  PubMed  CAS  Google Scholar 

  • Takato, M., and Goldring, S., 1979, Intracellular marking with lucifer yellow CH and horseradish peroxidase of cells electrophysiologically characterized as glia in the cerebral cortex of the cat, J. Comp. Neurol. 186:173–188.

    Article  PubMed  CAS  Google Scholar 

  • Tamamaki, N., Abe, K., and Nojyo, Y., 1987, Columnar organization in the subiculum formed by axon branches originating from single CA1 pyramidal neurons in the rat hippocampus, Brain Res. 412:156–160.

    Article  PubMed  CAS  Google Scholar 

  • Tamamaki, N., and Nojyo, Y., 1993, Projection of the entorhinal layer-II neurons in the rat as revealed by intracellular pressure-injection of neurobiotin, Hippocampus 3:471–480.

    Article  PubMed  CAS  Google Scholar 

  • Tepper, J. M., Sawyer, S. F., and Groves, P. M., 1987, Electrophysiologically identified nigral dopaminergic neurons intracellularly labeled with HRP: light-microscopic analysis, J. Neurosci. 7:2794–2806.

    PubMed  CAS  Google Scholar 

  • Thomas, R. C., and Wilson, V. J., 1965, Precise localization of Renshaw cells with a new marking technique, Nature 206:211–213.

    Article  PubMed  CAS  Google Scholar 

  • Wang, X. J., and Buzsaki, G., 1996, Gamma oscillation by synaptic inhibition in a hippocampal interneuronal network model, J. Neurosci. 16:6402–6413.

    PubMed  CAS  Google Scholar 

  • Wilson, M. A., and McNaughton, B. L., 1993, Dynamics of the hippocampal ensemble code for space, Science 261:1055–1058.

    Article  PubMed  CAS  Google Scholar 

  • Wise, K. D., and Najafi, K., 1991, Microfabrication techniques for integrated sensors and microsystems, Science 254:1335–1342.

    Article  PubMed  CAS  Google Scholar 

  • Ylinen, A., Soltesz, I., Bragin, A., Penttonen, M., Sik, A., and Buzsaki, G., 1995, Intracellular correlates of hippocampal theta rhythm in identified pyramidal cells, granule cells, and basket cells, Hippocampus 5:78–90.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre de Recherche Université Laval Robert-Giffard, 2601 De la Canardiere, Québec, Québec, G1J 2G3, Canada

    Attila Sík

Authors
  1. Attila Sík
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Center for Molecular and Behavioral Neuroscience, Rutgers University, Newark, NJ, USA

    Laszlo Zaborszky

  2. Department of Anatomy, Vrije University Medical Center, Amsterdam, The Netherlands

    Floris G. Wouterlood

  3. Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain

    José Luis Lanciego

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Springer Science+Business Media, Inc.

About this chapter

Cite this chapter

Sík, A. (2006). Merging Structure and Function: Combination of In Vivo Extracellular and Intracellular Electrophysiological Recordings with Neuroanatomical Techniques. In: Zaborszky, L., Wouterlood, F.G., Lanciego, J.L. (eds) Neuroanatomical Tract-Tracing 3. Springer, Boston, MA . https://doi.org/10.1007/0-387-28942-9_6

Download citation

Publish with us

Policies and ethics

Search

Navigation