Gepubliceerd in:
01-02-2013
Categorizing B-cell defects using an in-vitro B-cell differentiation assay
Auteurs:
Daan J. aan de Kerk, Machiel H. Jansen, Mirjam van der Burg, Ester M.M. van Leeuwen, Taco W. Kuijpers
Gepubliceerd in:
Tijdschrift voor Kindergeneeskunde
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bijlage 1/2013
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Extract
B-cells play an important role in primary immunodeficiencies, about 65% of cases show altered antibody production. Nevertheless many B-cell defects still have not been characterized in functional terms and at the genetic level. Hence, we need good tools to classify at which differentiation stage such a B-cell defect can be located.To identify and further characterize B cell immunodeficiencies we used an in-vitro assay comparing B-cell proliferation, differentiation and function for different known class-switch recombination (CSR) defective patients compared to patients with uncharacterized hypogammaglobulinemia. …